Overexpression of a tomato flavanone 3-hydroxylase-like protein gene improves chilling tolerance in tobacco
Overexpression of a tomato flavanone 3-hydroxylase-like protein gene improves chilling tolerance in tobacco
复制标题
番茄黄烷酮 3-羟化酶样蛋白基因的过度表达可提高烟草的耐冷性
DOI：
10.1016/j.plaphy.2015.08.019
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发表时间：
2015-11-01
影响因子：
6.5
通讯作者：
Kong, Fan-Ying
中科院分区：
文献类型：
--
作者：
Meng, Chen;Zhang, Song;Kong, Fan-Ying
类黄酮是植物中发现的次生代谢物，具有广泛的生物功能，例如应激保护。本研究利用转基因烟草研究了番茄（Solanum lycopersicum）黄烷酮 3-羟化酶样蛋白基因 SlF3HL 的功能。该基因的表达在寒冷（4℃）、热（42℃）、盐（NaCl）和氧化（H2O2）胁迫下上调。表现出高SIF3HL mRNA和蛋白质水平的转基因植物比野生型植物表现出更高的类黄酮含量。此外，三种类黄酮生物合成相关结构基因，即查耳酮合酶（CHS）、查耳酮异构酶（CHI）和黄酮醇合酶（FLS）的表达在转基因植物中也高于野生型植物。在寒冷胁迫下，与野生型植物相比，转基因植物不仅表现出更快的种子发芽、更好的存活和生长，而且还表现出较低的丙二醛（MDA）积累、相对电导率（REC）以及H2O2和O-2（中心点-）水平。这些结果表明，SlF3HL 刺激类黄酮生物合成以应对寒冷胁迫。 (C) 2015 Elsevier Masson SAS。版权所有。
Flavonoids are secondary metabolites found in plants with a wide range of biological functions, such as stress protection. This study investigated the functions of a tomato (Solanum lycopersicum) flavanone 3-hydroxylase-like protein gene SlF3HL by using transgenic tobacco. The expression of the gene was up-regulated under chilling (4 degrees C), heat (42 degrees C), salt (NaCl) and oxidative (H2O2) stresses. The transgenic plants that displayed high SIF3HL mRNA and protein levels showed higher flavonoid content than the WT plants. Moreover, the expression of three flavonoid biosynthesis-related structural genes, namely, chalcone synthase (CHS), chalcone isomerase (CHI) and flavonol synthase (FLS) was also higher in the transgenic plants than in the WT plants. Under chilling stress, the transgenic plants showed not only faster seed germination, better survival and growth, but also lower malondialdehyde (MDA) accumulation, relative electrical conductivity (REC) and H2O2 and O-2(center dot-) levels compared with WT plants. These results suggested that SlF3HL stimulated flavonoid biosynthesis in response to chilling stress. (C) 2015 Elsevier Masson SAS. All rights reserved.