Acute exercise increases adipose tissue interstitial adiponectin concentration in healthy overweight and lean subjects
Acute exercise increases adipose tissue interstitial adiponectin concentration in healthy overweight and lean subjects
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DOI：
10.1530/eje-07-0213
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发表时间：
2007-11-01
影响因子：
5.8
通讯作者：
Stallknecht, Bente
中科院分区：
文献类型：
--
作者：
Hojbjerre, Lise;Rosenzweig, Mary;Stallknecht, Bente
目的：研究急性运动对脂肪组织和血浆中脂联素的表达和浓度以及脂联素调节因子的影响。设计和方法：8名超重男性和8名瘦弱男性在南卡罗来纳州接受大孔微透析检查。取腹壁脂肪组织(SCAAT)，取动脉化血。其中一天休息3h，以最大摄氧量的55%运动1h，再休息2.5h，另一天休息6.5h，在包括运动的当天，在运动前后进行SCAAT检查。结果：运动增加了超重和消瘦受试者的SCAAT间质脂联素浓度，且不同组间浓度无差异。血浆脂联素在运动过程中没有增加，在超重和瘦的受试者中是相似的。SCAAT中脂联素mRNA在运动过程中下降，在超重和瘦的受试者中相似。令人惊讶的是，SCAAT的间质脂联素浓度仅为血浆浓度的20%。两组运动时SCAAT、IL-6微透析液和血浆浓度及SCAAT、IL-6mRNA均升高。在运动过程中，除SCAAT外，任何一组的血浆肿瘤坏死因子-α(TNF-α)浓度都没有变化。运动后两组大鼠脑组织中的mRNA表达均增加。此外，运动可降低SCAAT瘦素基因的表达，而抵抗素基因的表达无明显变化。结论：急性运动增加了超重和消瘦受试者的脂肪组织间质脂联素浓度，而血浆脂联素浓度没有明显变化。SCAAT间质中脂联素的浓度仅为血浆的20%。
Objective: We studied how an acute bout of exercise influences expression and concentration of adiponectin and regulators of adiponectin in adipose tissue and plasma. Design and methods: Eight overweight and eight lean males were examined by large-pore microdialysis in s.c. abdominal adipose tissue (SCAAT) and had arterialized blood sampled. On one day subjects rested for 3 h, exercised for I h at 55% of maximal oxygen uptake and rested again for 2.5 h, and on another day subjects rested for 6.5 h. On the day including exercise SCAAT was biopsied before and after exercise. Results: Exercise increased the SCAAT interstitial adiponectin concentration in both overweight and lean subjects and concentrations did not differ between groups. Plasma adiponectin did not increase during exercise and was similar in overweight and lean subjects. Adiponectin mRNA in SCAAT decreased during exercise and was similar in overweight and lean subjects. Surprisingly, the interstitial adiponectin concentration in SCAAT was only 20% of the plasma concentration. SCAAT interleukin-6 (IL-6) microdialyzate and plasma concentrations and SCAAT IL-6 mRNA increased during exercise in both groups. Tumor necrosis factor-a (TNF-alpha) plasma concentration did not change during exercise in any of the groups, but SCAAT TNF-alpha. mRNA increased after exercise in both groups. Furthermore, exercise decreased SCAAT leptin mRNA with no change in resistin mRNA. Conclusions: Acute exercise increases adipose tissue interstitial adiponectin concentration in both overweight and lean subjects with no major changes in plasma adiponectin concentration. The interstitial concentration of adiponectin in SCAAT is only 20% of that in plasma.