Determination of ractopamine in animal tissues by liquid chromatography-fluorescence and liquid chromatography/tandem mass spectrometry
Determination of ractopamine in animal tissues by liquid chromatography-fluorescence and liquid chromatography/tandem mass spectrometry
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DOI：
10.1016/s0003-2670(03)00120-x
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发表时间：
2003-04-25
影响因子：
6.2
通讯作者：
Hoffman, MK
中科院分区：
文献类型：
--
作者：
Shishani, E;Chai, SC;Hoffman, MK
建立了一种灵敏的方法，用于猪和牛肌肉组织中莱克多巴胺残留的常规筛选和确认。肌肉组织用甲醇提取，p -葡糖苷酶水解，液萃取，固相清理。采用荧光液相色谱法定量莱克多巴胺，以利托君为内标，串联质谱法测定莱克多巴胺含量。在1-4 ng g（-1）莱克多巴胺的条件下，莱克多巴胺在猪组织中的回收率为80 ~ 117%，在牛组织中的回收率为85 ~ 114%。本程序适用于监管机构规定的可食用肉类组织中莱克多巴胺残留的常规监测、筛选和确认。(C) 2003 Elsevier Science B.V.版权所有
A sensitive method was developed and validated for routine screening and confirmation of ractopamine residues in porcine and bovine muscle tissues. Muscle tissues were extracted with methanol and hydrolyzed with P-glucuronidase, followed by liquid extraction and solid-phase clean up. Ractopamine was quantified by liquid chromatography (LC) with fluorescence detection, using ritodrine as internal standard and confirmed by tandem mass spectrometry. Recoveries of ractopamine ranged between 80 and 117% in porcine tissues and 85 and 114% in bovine tissues at 1-4 ng g(-1) ractopamine. This procedure is suitable for routine monitoring, screening and confirmation of ractopamine residues in edible meat tissues, as mandated by regulatory agencies. (C) 2003 Elsevier Science B.V. All rights reserved.