Gelatin-based cell culture device for construction and X-ray irradiation of a three-dimensional oral cancer model
Gelatin-based cell culture device for construction and X-ray irradiation of a three-dimensional oral cancer model
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DOI：
10.1007/s44211-023-00308-6
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发表时间：
2023-02-27
影响因子：
1.6
通讯作者：
Sato, Kae
中科院分区：
文献类型：
--
作者：
Bessho, Tomoka;Takagi, Tomoko;Sato, Kae
使用三维（3D）组织模型的生物测定提供了优于2D培养测定的几个优点，因为它们可以再现天然组织的结构和功能。在这项研究中，我们使用我们新设计的明胶设备，以产生一个微型的三维模型的人口腔鳞状细胞癌的基质和血管。为了实现气液界面培养，我们设想了一种新的装置结构，其中三个威尔斯孔排列并通过分隔线分开;威尔斯孔可以通过去除分隔线连接。将细胞接种在具有分裂线的中心孔中以形成多层，然后在去除线后从侧威尔斯孔供应培养基。成功地将人口腔鳞状细胞癌（HSC-4）细胞、人脐静脉内皮细胞（HUVEC）和正常人真皮成纤维细胞（NHDF）共培养，产生了模拟3D癌症组织的结构。对该3D癌症模型进行X射线灵敏度测定，然后使用共聚焦显微镜和切片扫描电子显微镜评价DNA损伤。
Bioassays using three-dimensional (3D) tissue models offer several advantages over 2D culture assays because they can reproduce the structure and function of native tissues. In this study, we used our newly designed gelatin device to generate a miniature 3D model of human oral squamous cell carcinoma with stroma and blood vessels. To enable air-liquid interface culture, we conceived a new device structure in which three wells were lined up and separated by a dividing thread; the wells could be connected by removing the dividing thread. Cells were seeded in the center well with the dividing thread to form a multilayer, followed by the supply of media from the side wells after thread removal. Human oral squamous cell carcinoma (HSC-4) cells, human umbilical vein endothelial cells (HUVECs), and normal human dermal fibroblasts (NHDFs) were successfully cocultured, resulting in structures that mimicked 3D-cancer tissues. This 3D-cancer model was subjected to an X-ray sensitivity assay, followed by the evaluation of DNA damage using confocal microscopy and section-scanning electron microscopy.