Effects of Different Fatty Acid Chain Lengths on Fatty Acid Oxidation-Related Protein Expression Levels in Rat Skeletal Muscles
Effects of Different Fatty Acid Chain Lengths on Fatty Acid Oxidation-Related Protein Expression Levels in Rat Skeletal Muscles
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DOI：
10.5650/jos.ess14199
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发表时间：
2015-04-01
影响因子：
1.5
通讯作者：
Nakatani, Akira
中科院分区：
文献类型：
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作者：
Ishizawa, Rie;Masuda, Kazumi;Nakatani, Akira
骨骼肌可以适应影响能量代谢的饮食干预。中链脂肪酸（MCFA）的膳食摄入增强了Ha型骨骼肌纤维中脂肪酸（FAO）的线粒体氧化。然而，MCFA饮食对不同类型肌纤维中线粒体或细胞质FAO相关蛋白表达水平的影响仍不清楚。本研究旨在研究高脂饮食，MCFA，对线粒体酶活性和心脏型脂肪酸结合蛋白（H-FABP）水平在不同类型的骨骼肌纤维的影响。将5周龄雄性Wistar大鼠分配至以下三种饮食条件之一：标准食物（SC，12%的热量来自脂肪）、高脂肪MCFA或高脂肪长链脂肪酸（LCFA）饮食（两者均60%的热量来自脂肪）。动物自由进食和饮水4周，随后分析柠檬酸合酶（CS）活性和H-FABP浓度。MCFA组的附睾脂肪垫（EFP）明显小于LCFA组（p < 0.05）。与SC喂养对照组相比，MCFA喂养组在所有类型的骨骼肌纤维（三头肌、腓肠肌表面部分（gasS）、腓肠肌深部（gasD）和比目鱼肌; p < 0.05）中显示CS活性增加。LCFA组中的H-FABP浓度显著高于SC喂养组和MCFA喂养组（三头肌、gasS、gasD和比目鱼肌; p < 0.05）。然而，在SC喂养组和MCFA喂养组之间未观察到H-FABP浓度的显著差异。这项研究的结果表明，MCFA饮食可以增加线粒体酶CS的表达，但不是H-FABP的表达，在快肌和慢肌纤维中，这表明H-FABP的表达依赖于骨骼肌细胞胞质中脂肪酸的链长。
Skeletal muscles can adapt to dietary interventions that affect energy metabolism. Dietary intake of medium-chain fatty acids (MCFAs) enhances mitochondrial oxidation of fatty acids (FAO) in type Ha skeletal muscle fibers. However, the effect of MCFAs diet on mitochondrial or cytoplasmic FAO-related protein expression levels in different types of muscle fibers remains unclear. This study aims to examine the effects of a high-fat diet, containing MCFAs, on mitochondrial enzyme activities and heart-type fatty acid-binding protein (H-FABP) levels in different types of skeletal muscle fibers. Five-week-old male Wistar rats were assigned to one of the following three dietary conditions: standard chow (SC, 12% of calories from fat), high-fat MCFA, or high-fat long-chain fatty acids (LCFAs) diet (60% of calories from fat for both). The animals were provided food and water ad libitum for 4 weeks, following which citrate synthase (CS) activity and H-FABP concentration were analyzed. The epididymal fat pads (EFP) were significantly smaller in the MCFA group than in the LCFA group (p < 0.05). MCFA-fed group displayed an increase in CS activity compared with that observed in SC-fed controls in all types of skeletal muscle fibers (triceps, surface portion of gastrocnemius (gasS), deep portion of gastrocnemius (gasD), and soleus; p < 0.05,). H-FABP concentration was significantly higher in the LCFA group than in both the SC-fed and MCFA-fed groups (triceps, gasS, gasD, and soleus; p < 0.05,). However, no significant difference was observed in the H-FABP concentrations between the SC-fed and MCFA-fed groups. The results of this study showed that the MCFA diet can increase the expression of the mitochondrial enzyme CS, but not that of H-FABP, in both fast- and slow-twitch muscle fibers, suggesting that H-FABP expression is dependent on the chain length of fatty acids in the cytoplasm of skeletal muscles cells.