Optical fiber immunosensor based on a poly(pyrrole-benzophenone) film for the detection of antibodies to viral antigen
Optical fiber immunosensor based on a poly(pyrrole-benzophenone) film for the detection of antibodies to viral antigen
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DOI：
10.1021/ac048569w
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发表时间：
2005-03-15
影响因子：
7.4
通讯作者：
Marks, RS
中科院分区：
文献类型：
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作者：
Konry, T;Novoa, A;Marks, RS
在这里，我们描述了一种新开发的用于丙型肝炎病毒(HCV)诊断的光学微生物传感器，该传感器使用一种新的光固定方法，该方法基于沉积在表面导电光纤上的可光激活的电生聚合物膜，然后使用该膜通过光中介将生物感受器连接到光纤尖端。这种光纤导电表面的改性是通过在光纤的二氧化硅表面沉积一层薄薄的氧化铟锡来完成的。然后将单体电聚合到导电金属氧化物表面；然后，将纤维浸入含有丙型肝炎病毒E2包膜蛋白抗原的溶液中，并用紫外光(波长类似于345 nm)照明。作为光化学反应的结果，一层薄薄的抗原成为共价结合到二苯甲酮修饰的表面上。光化学修饰的光纤作为免疫传感器用于检测通过化学发光反应检测的抗E2蛋白抗体分析物。对该生物传感器进行了灵敏度、特异度和总体实用性测试。我们的结果表明，利用该微生物传感器检测抗E2抗体可以显著提高丙型肝炎病毒的血清学标准检测，特别是在标准免疫学试验诊断为丙型肝炎病毒阴性但已知携带病毒的透析患者中。如果转化为一种简单易用的方法，该方法可能在未来作为一种重要的临床工具用于血库中的丙型肝炎筛查。
We describe herein a newly developed optical microbiosensor for the diagnosis of hepatitis C virus (HCV) by using a novel photoimmobilization methodology based on a photoactivable electrogenerated polymer film deposited upon surface-conductive fiber optics, which are then used to link a biological receptor to the fiber tip through light mediation. This fiber-optic electroconductive surface modification is done by the deposition of a thin layer of indium tin oxide on the silica surface of the fiber optics. Monomers are then electropolymerized onto the conductive metal oxide surface; thereafter, the fibers are immersed in a solution containing HCV-E2 envelope protein antigen and illuminated with UV light (wavelength similar to 345 nm). As a result of the photochemical reaction, a thin layer of the antigen becomes covalently bound to the benzophenone-modified surface. The photochemically modified fiber optics were tested as immunosensors for the detection of anti-E2 protein antibody analyte that was measured through chemiluminescence reaction. The biosensor was tested for sensitivity, specificity, and overall practicality. Our results suggest that the detection of anti-E2 antibodies with this microbiosensor may enhance significantly HCV serological standard testing especially among patients during dialysis, which were diagnosed as HCV negative, by standard immunological tests, but were known to carry the virus. If transformed into an easy to use procedure, this assay might be used in the future as an important clinical tool for HCV screening in blood banks.