Image Reconstruction of the Subbasal Nerve Plexus with In Vivo Confocal Microscopy
Image Reconstruction of the Subbasal Nerve Plexus with In Vivo Confocal Microscopy
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DOI：
10.1167/iovs.10-6065
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发表时间：
2011-08-01
影响因子：
4.4
通讯作者：
Stachs, Oliver
中科院分区：
文献类型：
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作者：
Allgeier, Stephan;Zhivov, Andrey;Stachs, Oliver
目的.为了克服活体共聚焦激光扫描显微镜（CLSM）中的角膜前镶嵌（ACM）现象，重建基底下神经丛（SNP）的无失真图像，以便于在ACM脊存在的情况下进行形态计量学分析。在5名健康志愿者中进行CLSM。提出了一种基于相位相关的图像处理算法，用于分析和消除体扫描图像序列中的运动失真。对SNP进行三维追踪以重建仅包含SNP层的图像，即使在ACM区域也可以清晰地看到神经纤维。SNP的实时绘图显示在所有情况下都存在具有K结构的脊。K结构的出现与裂隙灯观察到的ACM的发展直接相关，并导致Bowman膜水平的大量变形，严重干扰SNP结构的检查。ACM山脊的平均海拔为20.6 μ m（范围为8.7-34.0 μ m）。新方法允许在ACM区域重建SNP层。所描述的方法允许精确分析和消除CLSM体积扫描中的运动伪影，结合即使在存在严重ACM的情况下也能够重建SNP结构的能力。所描述的算法的鲁棒性和自动化需要持续的开发，但这将为角膜神经再生或变性的扩展研究以及用于临床实践提供良好的基础。（Invest Ophthalmol维斯科学。2011; 52：5022-5028）DOI：10.1167/iovs.10-6065
PURPOSE. To overcome the anterior corneal mosaic (ACM) phenomenon in in vivo confocal laser scanning microscopy (CLSM) and to reconstruct undistorted images of the subbasal nerve plexus (SNP), facilitating morphometric analysis in the presence of ACM ridges.METHODS. CLSM was performed in five healthy volunteers. An original image processing algorithm based on phase correlation was used to analyze and reduce motion distortions in volume scan image sequences. Three-dimensional tracing of the SNP was performed to reconstruct images containing only the SNP layer, with nerve fibers clearly visible even in ACM areas.RESULTS. Real-time mapping of the SNP revealed the presence of ridges with K-structures underneath them in all cases. The occurrence of K-structures correlated directly with development of ACM observed by slit lamp and resulted in massive deformation at the level of Bowman's membrane, seriously interfering with examination of SNP structures. The average elevation of ACM ridges was 20.6 mu m (range, 8.7-34.0 mu m). The novel method presented permitted reconstruction of the SNP layer in regions of ACM.CONCLUSIONS. The described method allows the precise analysis and elimination of motion artifacts in CLSM volume scans, in conjunction with the capability to reconstruct SNP structures even in the presence of severe ACM. The robustness and automation of the described algorithms require ongoing development, but this will provide a sound basis for extended studies of corneal nerve regeneration or degeneration and for use in clinical practice. (Invest Ophthalmol Vis Sci. 2011; 52: 5022-5028) DOI: 10.1167/iovs.10-6065