Biorefinery of galacturonic acid using a biofuel cell as a reactor
Biorefinery of galacturonic acid using a biofuel cell as a reactor
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使用生物燃料电池作为反应器的半乳糖醛酸生物精炼厂
DOI：
10.1039/d2re00202g
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发表时间：
2022
影响因子：
3.9
通讯作者：
Nobuhumi Nakamura
中科院分区：
文献类型：
--
作者：
Tomoe Nakagawa; Hayato Abe; Tomoko Gessei; Kouta Takeda; Kiyohiko Igarashi; Nobuhumi Nakamura
构建了基于酶生物燃料电池的反应器（EBFC反应器），以同时从生物质中产生电力和化学产品。生物质以前被用作化学产品的原材料或作为能源。为了同时获得能量和产物材料，我们进行了酶促反应，其中吡咯喹啉醌依赖性葡萄糖脱氢酶（PQQ-GDH）将半乳糖醛酸（果胶的主要成分，生物质）氧化为半乳糖醛酸。在该EBFC反应器中，阳极由物理吸附在涂有聚亚甲基绿色的碳毡（CF）上的PQQ-GDH组成，阴极由物理吸附在裸CF上的胆红素氧化酶（BOD）组成。首先，通过用恒电位仪保持阳极电位恒定，确认在PQQ-GDH修饰电极上进行酶反应，其中在24小时内以93%的产率获得半乳糖苷酸而没有副产物。第二，将生物阳极与BOD改性的生物阴极组合以构建EBFC反应器。开路电压为0.58 ± 0.07 V，负载电阻为4 kΩ时，最大功率为90 ± 7 μW cm−3。EBFC的转化率为3.1%。因此，它被证明，EBFC，这已被公认为一个系统，用于获得能量，也可以用作生物精炼反应器。
A reactor based on an enzymatic biofuel cell (an EBFC reactor) was constructed to simultaneously generate electricity and chemical products from biomass. Biomass has previously been used either as a raw material for chemical products or as an energy source. To obtain energy and product materials at the same time, we performed an enzymatic reaction in which pyrroloquinoline-quinone-dependent glucose dehydrogenase (PQQ-GDH) oxidizes galacturonic acid (the main component of pectin, a biomass) to galactaric acid. In this EBFC reactor, the anode consisted of PQQ–GDH physically adsorbed on carbon felt (CF) coated with poly-methylene green and the cathode consisted of bilirubin oxidase (BOD) physisorbed onto bare CF. First, the enzymatic reaction was confirmed to proceed at the PQQ–GDH-modified electrode by keeping the anode potential constant by a potentiostat, where galactaric acid was obtained in a 93% yield without byproducts in 24 hours. Second, the bioanode was combined with a BOD-modified biocathode to build an EBFC reactor. The open-circuit voltage was 0.58 ± 0.07 V, and the maximum power was 90 ± 7 μW cm−3 for a load resistance of 4 kΩ. The conversion yield of EBFC was 3.1%. Thus, it was demonstrated that the EBFC, which has been recognized as a system for obtaining energy, can also be used as a biorefinery reactor.