Enhancement of viral transformation for evaluation of the carcinogenic or mutagenic potential of inorganic metal salts.
Enhancement of viral transformation for evaluation of the carcinogenic or mutagenic potential of inorganic metal salts.
复制标题
增强病毒转化以评估无机金属盐的致癌或致突变潜力。
作者：
B. Casto;J. Meyers;J. DiPaolo
38种金属盐被测试了它们通过猿腺病毒SA7促进叙利亚仓鼠胚胎细胞转化的能力。所有已知具有动物致癌潜力或对微生物或哺乳动物细胞具有诱变活性的金属盐都能增加SA7的转化频率。根据产生显著增强所需的浓度，将金属分为三组。那些表现出最高活性(小于0.05 mm的正极)的是锑、砷、镉、铬和铂的盐类。第二组(正数为0.05-0.6 mm)包括铍、钴、铜、铅、锰、汞、镍、银、铊和锌。将铁盐放入第三组(仅在浓度大于0.9 mM时呈阳性)。除氯化锌和硫酸锌外，处理细胞中病毒转化频率的相对增加和转化灶数目的绝对增加都显示出增强作用。后一种观察和在没有显性细胞杀伤的情况下增强的证明否定了选择转化敏感细胞导致增强的可能性。
Thirty-eight metal salts were tested for their capacity to enhance transformation of Syrian hamster embryo cells by a simian adenovirus, SA7. All of the metal salts with known carcinogenic potential in animals or mutagenic activity in microbial or mammalian cells increased the SA7 transformation frequency. Metals were classified into three groups according to the concentration necessary to produce significant enhancement. Those showing highest activity (positive at less than 0.05 mM) were the salts of antimony, arsenic, cadmium, chromium, and platinum. The second group (positive from 0.05 to 0.6 mM) included beryllium, cobalt, copper, lead, manganese, mercury, nickel, silver, thallium, and zinc. Iron salts were placed in a third group (only positive at concentrations greater than 0.9 mM). With the exception of ZnCl2 and ZnSO4, enhancement was demonstrated by both a relative increase in the viral transformation frequency and an absolute increase in the number of transformed foci among treated cells. The latter observation and the demonstration of enhancement in the absence of overt cell killing negate the possibility that enhancement resulted from the selection of transformation-sensitive cells.