Bacterial entry to the splenic white pulp initiates antigen presentation to CD8+ T cells
Bacterial entry to the splenic white pulp initiates antigen presentation to CD8+ T cells
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DOI：
10.1016/j.immuni.2008.06.013
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发表时间：
2008-09-19
期刊：
影响因子：
32.4
通讯作者：
Miller, Mark J.
中科院分区：
文献类型：
--
作者：
Aoshi, Taiki;Zinselmeyer, Bernd H.;Miller, Mark J.
脾脏在宿主对细菌的保护反应中起着重要作用。然而，导致病原体特异性免疫的细胞动力学仍然知之甚少。本研究通过原位荧光显微镜检测小鼠脾脏单核细胞增生李斯特菌（Lm）感染。我们发现，Lm从边缘区（MZ）到动脉周围淋巴鞘（PALS）的再分配受到百日咳毒素的抑制，并且需要CD11c（+）细胞的存在。早在感染后9小时，我们通过双光子显微镜检测到PALS外周区域感染的树突状细胞和lm特异性T细胞聚集。百日咳毒素抑制Lm进入PALS和抗原呈递到CD8(+) T细胞。我们的研究表明，脾树突状细胞迅速将胞内细菌传递到白髓的T细胞区域，启动CD8(+) T细胞反应。
The spleen plays an important role in host-protective responses to bacteria. However, the cellular dynamics that lead to pathogen-specific immunity remain poorly understood. Here we examined Listeria monocytogenes (Lm) infection in the mouse spleen via in situ fluorescence microscopy. We found that the redistribution of Lm from the marginal zone (MZ) to the periarteriolar lymphoid sheath (PALS) was inhibited by pertussis toxin and required the presence of CD11c(+) cells. As early as 9 hr after infection, we detected infected dendritic cells in the peripheral regions of the PALS and clustering of Lm-specific T cells by two-photon microscopy. Pertussis toxin inhibited both Lm entry into the PALS and antigen presentation to CD8(+) T cells. Our study suggests that splenic dendritic cells rapidly deliver intracellular bacteria to the T cell areas of the white pulp to initiate CD8(+) T cell responses.