Functional analysis of chick ONT1 reveals distinguishable activities among olfactomedin-related signaling factors
Functional analysis of chick ONT1 reveals distinguishable activities among olfactomedin-related signaling factors
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DOI：
10.1016/j.mod.2005.11.005
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发表时间：
2006-02-01
影响因子：
2.6
通讯作者：
Sasai, Y
中科院分区：
文献类型：
--
作者：
Sakuragi, M;Sasai, N;Sasai, Y
嗅觉介蛋白家族是一类相对较新的细胞外蛋白。两个家族成员已被证明在外胚层组织的早期发育中发挥作用：Noelin增强小鸡的神经嵴生成，Tiarin促进爪蟾的背神经特化。在这项研究中，我们介绍了一个新的成员的嗅觉调节蛋白家族，ONT 1。在早期鸡胚中，ONT 1的表达首先出现在亨森结，随后在轴向和近轴中胚层。当神经管关闭时，ONT 1的强表达在从中脑嘴侧到后脑的顶板区域中被短暂地发现。ONT 1在这些区域的过表达以类似于Noelin的方式延长了神经嵴细胞的产生。有趣的是，ONT和Noelin对鸡中迁移神经嵴标记物HNK-1的表达具有相反的作用：它们分别引起该标记物的抑制和异位诱导。嗅觉调节素相关因素之间的差异活动进一步研究在非洲爪蟾。显微注射到非洲爪蟾胚胎中的ONT mRNA扩大了神经嵴标记物FoxD 3在神经胚阶段的表达域，而Tiarin或Noelin的过表达抑制了FoxD 3。ONT对爪蟾神经管没有表现出背侧化作用，这与Tiarin所见的强背侧化活性形成对比。因此，即使在相同的实验系统中，不同的Olfactomedin相关因子也会引起不同的表型，这表明Olfactomedin家族在胚胎发生中使用多个响应系统来介导其信号。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
The Olfactomedin family is a relatively new class of extracellular proteins. Two family members have been shown to play roles in the early development of ectodermal tissues: Noelin enhances neural crest generation in chick and Tiarin promotes dorsal neural specification in Xenopus. In this study, we introduce a novel member of the Olfactomedin family, ONT1. In the early chick embryo, ONT1 expression first appears at Hensen's node and subsequently in the axial and paraxial mesoderm. When the neural tube closes, strong expression of ONT1 is transiently found in the roof plate region from the rostral midbrain to the hindbrain. Overexpression of ONT1 in these regions prolongs the generation of neural crest cells in a manner similar to that of Noelin. Interestingly, ONTI and Noelin have opposing effects on the expression of the migrating neural crest marker HNK-1 in the chick: they, respectively, cause suppression and ectopic induction of this marker. Differential activities among Olfactomedin-related factors are further examined in Xenopus. Microinjection of ONTI mRNA into the Xenopus embryo expands the expression domain of the neural crest marker FoxD3 at the neurula stage whereas overexpression of Tiarin or Noelin suppresses FoxD3. ONTI exhibits no dorsalizing effects on the Xenopus neural tube, which contrasts with the strong dorsalizing activity seen for Tiarin. Thus, distinct Olfactomedin related factors evoke qualitatively different phenotypes even in the same experimental systems, suggesting that Olfactomedin family uses multiple response systems to mediate its signals in embryogenesis. (c) 2005 Elsevier Ireland Ltd. All rights reserved.