A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization
A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization
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DOI：
10.1101/gad.1189004
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发表时间：
2004-06-15
影响因子：
10.5
通讯作者：
Singer, RH
中科院分区：
文献类型：
--
作者：
Gu, W;Deng, YF;Singer, RH
在酵母中，决定交配型转换的蛋白决定因子Ash1p在子细胞核内分离，建立HO表达的不对称。Ash1p的积累是由于ASH1 mRNA作为核糖核蛋白颗粒（mRNP或位点体）被分类到芽的远端顶端，在那里发生翻译。为了研究调节ASH1 mRNA翻译的机制，我们分离了ASH1位点体，并通过MALDI-TOF对相关蛋白进行了表征。其中一种蛋白是Puf6p，它是高度保守的rna结合蛋白PUF家族的新成员，如果蝇中的Pumilio，负责翻译抑制，通常影响不对称表达。puf6p结合的PUF一致序列位于ASH1 mRNA的TUTR中，并在体内和体外抑制ASH1 mRNA的翻译。在puf6Delta菌株中，Ash1p和ASH1 mRNA的不对称定位明显减少。我们认为Puf6p是一种控制ASH1 mRNA翻译的蛋白，这种翻译抑制在定位之前是必要的。
In yeast Saccharomyces cerevisiae, Ash1p, a protein determinant for mating-type switching, is segregated within the daughter cell nucleus to establish asymmetry of HO expression. The accumulation of Ash1p results from ASH1 mRNA that is sorted as a ribonucleoprotein particle (mRNP or locasome) to the distal tip of the bud where translation occurs. To study the mechanism regulating ASH1 mRNA translation, we isolated the ASH1 locasome and characterized the associated proteins by MALDI-TOF. One of these proteins was Puf6p, a new member of the PUF family of highly conserved RNA-binding proteins such as Pumilio in Drosophila, responsible for translational repression, usually to effect asymmetric expression. Puf6p-bound PUF consensus sequences in the TUTR of ASH1 mRNA and repressed the translation of ASH1 mRNA both in vivo and in vitro. In the puf6Delta strain, asymmetric localization of both Ash1p and ASH1 mRNA were significantly reduced. We propose that Puf6p is a protein that functions in the translational control of ASH1 mRNA, and this translational inhibition is necessary before localization can proceed.