Label-Free Single-Molecule Imaging with Numerical-Aperture-Shaped Interferometric Scattering Microscopy.
Label-Free Single-Molecule Imaging with Numerical-Aperture-Shaped Interferometric Scattering Microscopy.
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DOI：
10.1021/acsphotonics.6b00912
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发表时间：
2017-02-15
期刊：
影响因子：
7
通讯作者：
Kukura P
中科院分区：
文献类型：
--
作者：
Cole D;Young G;Weigel A;Sebesta A;Kukura P
我们光学询问纳米级物体的能力是由它们的消光截面和衍射限制区域之间的差异控制的，光可以被限制在远场。我们表明，放置在一个高数值孔径显微镜物镜的后焦平面附近的部分透射空间掩模增强消光对比度的散射体附近的接口由约T-1/2，其中T是transmands的掩模。基于数值孔径的背景与散射光的区分代表了增加消光对比度的一般方法，并使常规无标记成像下降到单分子水平。
Our ability to optically interrogate nanoscopic objects is controlled by the difference between their extinction cross sections and the diffraction-limited area to which light can be confined in the far field. We show that a partially transmissive spatial mask placed near the back focal plane of a high numerical aperture microscope objective enhances the extinction contrast of a scatterer near an interface by approximately T–1/2, where T is the transmissivity of the mask. Numerical-aperture-based differentiation of background from scattered light represents a general approach to increasing extinction contrast and enables routine label-free imaging down to the single-molecule level.