Cellular Dynamics of Memory B Cell Populations: IgM+ and IgG+ Memory B Cells Persist Indefinitely as Quiescent Cells.
Cellular Dynamics of Memory B Cell Populations: IgM+ and IgG+ Memory B Cells Persist Indefinitely as Quiescent Cells.
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记忆B细胞群体的细胞动力学：IgM+和IgG+存储B细胞无限期地作为静态细胞。
DOI：
10.4049/jimmunol.1501365
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发表时间：
2015-11-15
期刊：
影响因子：
--
通讯作者：
Allman D
中科院分区：
文献类型：
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作者：
Jones DD;Wilmore JR;Allman D
尽管记忆B细胞在长期免疫中起着关键作用，但它们的寿命仍然很难确定。使用四环素调节的脉冲追踪系统，我们测量了402天内预先建立的抗原诱导免疫球蛋白（IG）类别转换和IgM阳性记忆B细胞的群体周转率和个体半衰期。我们的研究结果表明，一旦建立，IgG阳性和不太频繁的IgM阳性记忆人群是非常稳定的，几乎没有证据的磨损或细胞周转。事实上，两个池中的绝大多数细胞显示出似乎超过小鼠寿命的半衰期，与成熟的幼稚B细胞形成鲜明对比。这些结果表明，回忆抗体反应介导的非常长寿的细胞的稳定池，并表明，抗原经验的B细胞采用非常有效的生存机制。
Despite their critical role in long-term immunity, the lifespan of individual memory B cells remains poorly defined. Using a tetracycline-regulated pulse-chase system, we measured population turnover rates and individual half-lives of pre-established antigen-induced immunoglobulin (Ig) class-switched and IgM-positive memory B cells over 402 days. Our results indicate that, once established, both IgG-positive and less frequent IgM-positive memory populations are exceptionally stable, with little evidence of attrition or cellular turnover. Indeed, the vast majority of cells in both pools exhibited half-lives that appear to exceed the lifespan of the mouse, contrasting dramatically with mature naïve B cells. These results indicate that recall antibody responses are mediated by stable pools of extremely long-lived cells, and suggest that antigen-experienced B cells employ remarkably efficient survival mechanisms.