Oligoarginine vectors for intracellular delivery: Design and cellular-uptake mechanisms
Oligoarginine vectors for intracellular delivery: Design and cellular-uptake mechanisms
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DOI：
10.1002/bip.20421
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发表时间：
2006-01-01
期刊：
影响因子：
2.9
通讯作者：
Futaki, S
中科院分区：
文献类型：
--
作者：
Futaki, S
利用膜渗透性肽载体的细胞内递送是最近发展起来的一种方法，已成功地将各种生物活性分子转运到细胞中以改变细胞功能。有效的递送蛋白质，多肽，核酸，脂质体，等等已经完成使用这种方法结合肽载体与货物分子。这种方法在医疗和制药应用方面的潜力也引起了我们的注意。富含精氨酸的肽，包括源自人类免疫缺陷病毒I型（HIV-I） Tat蛋白的基本肽段，被归类为最常用的肽载体之一。设计新型载体的努力一直在进行中。这些肽的内化以前被认为不采用内吞作用。然而，最近的重新评估已经证明了内吞作用在这些肽的细胞摄取中的重要作用。这些富含精氨酸的肽载体在内化方面有许多共同的特点。然而，似乎在这些多肽的内化模式中同时观察到某些不同之处。本文综述了这些富含精氨酸的肽载体的结构特征，并对其内化机制进行了综述。(c) 2005 Wiley期刊有限公司
Intracellular delivery using membrane-permeable peptide vectors is a recently developed methodology that has been employed successfully to transport various bioactive molecules into cells to modify cell functions. The efficient delivery of proteins, peptides, nucleic acids, liposomes, and so on has been accomplished using this methodology by conjugation of a peptide vector with the cargo molecules. The potentials of this approach for medical and pharmaceutical applications has also attracted our attention. Arginine-rich peptides, including a basic peptide segment derived from the human immunodeficiency virus type I (HIV-I) Tat protein, are categorized into one of the most frequently used peptide vectors. and the efforts of designing novel vectors have been ongoing. Internalization of these peptides has previously been regarded as not employing endocytosis. However, recent reevaluations have demonstrated the significant involvement of endocytosis in the cellular uptake of these peptides. These arginine-rich peptide vectors share many common features in internalization. However, there seem to be certain simultaneous dissimilarities observed in the modes of internalization among these peptides. In this review, the structural features of these arginine-rich peptide vectors have been focused on and the current understandings of their internalization mechanisms are summarized. (c) 2005 Wiley Periodicals, Inc.