Distribution of mitochondria in reconstructed mouse oocytes
Distribution of mitochondria in reconstructed mouse oocytes
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DOI：
10.1530/rep.1.00093
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发表时间：
2004-02-01
期刊：
影响因子：
3.8
通讯作者：
Fulka, H
中科院分区：
文献类型：
--
作者：
Fulka, H
有人建议，细胞核置换（转移）可以作为一种有效的卵母细胞治疗，以防止突变的线粒体DNA从母亲传给后代的人类。重要的和尚未回答的问题是，核质周围的线粒体将如何分布在新重建的卵母细胞。在我们的模型实验中，我们已经评估了线粒体的分布在重建的未成熟小鼠卵母细胞时，germinal囊泡核质体，与标记的线粒体，融合到未标记的胞质体。在融合开始后，几乎可以立即检测到线粒体从核质进入胞质。在未成熟的重构卵母细胞中，线粒体首先位于卵母细胞中心，但在成熟完成之前，它们均匀地分布在整个细胞质中。不同成熟阶段卵母细胞的融合表明线粒体分布的速度与细胞周期有关。
It has been suggested that nucleus replacement (transfer) may be used as an efficient oocyte therapy in order to prevent transmission of mutated mitochondrial DNA from mother to offspring in humans. The essential and not yet answered question is how mitochondria surrounding the karyoplast will be distributed in the newly reconstructed oocytes. In our model experiments, we have evaluated the distribution of mitochondria in reconstructed immature mouse oocytes when germinal vesicle karyoplasts, with labeled mitochondria, were fused to unlabeled cytoplasts. The penetration of mitochondria from karyoplasts into cytoplasts can be detected almost immediately after the beginning of fusion. In immature reconstructed oocytes, mitochondria are first located in the oocyte center but they are homogenously distributed within the whole cytoplasm before the completion of maturation. Fusion of oocytes at different stages of maturation suggests that the speed of mitochondria distribution is cell cycle dependent.