A novel chitin-binding protein identified from the peritrophic membrane of the cabbage looper, Trichoplusia ni
A novel chitin-binding protein identified from the peritrophic membrane of the cabbage looper, Trichoplusia ni
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DOI：
10.1016/j.ibmb.2005.06.003
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发表时间：
2005-11-01
影响因子：
3.8
通讯作者：
Wang, P
中科院分区：
文献类型：
--
作者：
Guo, W;Li, GX;Wang, P
从卷心菜环纹夜蛾中鉴定出一种新的中肠周膜蛋白TnPM-P42。经SDS-PAGE分析，TnPM-P42蛋白大小为42 kDa，与PM全长相关。在弓形虫幼虫取食期间，中肠是唯一可检测到TnPM-P42的组织。TnPM-P42具有几丁质结合活性，并与PM密切相关，这与目前已知的周酚型PM蛋白相似。然而，TnPM-P42代表了一个独特的蛋白质家族，在其序列特征上明显不同于周酚型PM蛋白。TnPM-P42不包含目前已知的所有PM蛋白中都存在的周罗芬结构域，而是具有几丁质脱乙酰酶样结构域。对GenBank数据库的序列相似性搜索没有鉴定出与TnPM-P42具有显著总体序列相似性的任何已知蛋白质。然而，来自不同节肢动物的表达序列标签(EST)被鉴定为编码与TnPM-P42具有高度序列相似性的蛋白质，表明TnPM-P42在其他节肢动物中存在同源物。与从节肢动物中鉴定出的各种EST一致，Western印迹分析表明，在绿色日光夜蛾和玉米夜蛾幼虫的PM中存在TnPM-P42样蛋白。TnPM-P42的序列特征表明，TnPM-P42代表了一个新的昆虫蛋白家族。然而，其生化和生理功能有待进一步研究。(C)2005爱思唯尔有限公司。保留所有权利。
A novel midgut peritrophic membrane (PM) protein, TnPM-P42, was identified from the cabbage looper, Trichoplusia ni. TnPM-P42 was shown as a 42 kDa protein by SDS-PAGE analysis and appeared to be associated with the PM throughout its entire length. In T. ni larvae, the midgut is the only tissue where TnPM-P42 could be detected during the feeding period of the larvae. TnPM-P42 has chitin-binding activity and is strongly associated with the PM, which is similar to the currently known peritrophin type PM proteins. However, TnPM-P42 represents a unique family of proteins distinctly different from the peritrophin type PM proteins in its sequence characteristics. TnPM-P42 does not contain the peritrophin domain which is present in all the currently known PM proteins, but instead has a chitin deacetylase-like domain. Sequence similarity search of the GenBank database did not result in identification of any known proteins with a significant overall sequence similarity to the TnPM-P42. However, expressed sequence tags (ESTs) from various arthropods were identified to code for proteins with high sequence similarities to TnPM-P42, indicating the presence of TnPM-P42 homologs in other arthropods. Consistent with the identification of various ESTs from arthropods, Western blot analysis demonstrated the presence of a TnPM-P42-like protein in the PMs from Heliothis virescens and Helicoverpa Zea larvae. The sequence characteristics of TnPM-P42 indicate that TnPM-P42 represents a novel family of insect proteins. However, its biochemical and physiological functions require further investigation. (c) 2005 Elsevier Ltd. All rights reserved.