Molecular cytogenetic evaluation of chromosome instability in Triticum aestivum-Secale cereale disomic addition lines
Molecular cytogenetic evaluation of chromosome instability in Triticum aestivum-Secale cereale disomic addition lines
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DOI：
10.1007/bf03195723
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发表时间：
2010-01-01
影响因子：
2.4
通讯作者：
Molnar-Lang, M.
中科院分区：
文献类型：
--
作者：
Szakacs, E.;Molnar-Lang, M.
采用Feulgen法和荧光原位杂交（FISH）检测了小麦/黑麦（‘中国春’/‘皇’）二体附加系的遗传稳定性。Feulgen染色在二体附加系的后代中检测到不同比例的二体、单体和端粒植物。7R添加系稳定性最好，而2R和4R添加系最不稳定。用FISH检测染色体重排。根据重复DNA探针pSc119.2和（AAC）(5)以及核糖体DNA探针（5S和45S）的特异性杂交模式，鉴定了1R和4R附加系后代的同工染色体。这些结果引起了人们对使用FISH对基本遗传物质进行连续细胞学检查的重要性的关注，因为这种方法可以揭示传统Feulgen染色技术或分子标记无法检测到的染色体重排。
The genetic stability of wheat/rye ('Chinese Spring'/'Imperial') disomic addition lines was checked using the Feulgen method and fluorescent in situ hybridization (FISH). Feulgen staining detected varying proportions of disomic, monosomic, and telosomic plants among the progenies of the disomic addition lines. The greatest stability was observed for the 7R addition line, while the most unstable lines were those with 2R and 4R additions. Chromosome rearrangements were also detected using FISH. Based on the specific hybridization patterns of repetitive DNA probes pSc119.2 and (AAC)(5), as well as ribosomal DNA probes (5S and 45S), isochromosomes were identified in the progenies of 1R and 4R addition lines. The results draw attention to the importance of continuous cytological checks on basic genetic materials by using FISH, because this method reveals chromosome rearrangements that could not be detected either with the conventional Feulgen staining technique or with molecular markers.