A simple method of preparing myosin from porcine aortae.
A simple method of preparing myosin from porcine aortae.
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一种从猪主动脉制备肌球蛋白的简单方法。
DOI：
10.1080/00327488708062473
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Driska,SP
中科院分区：
文献类型：
--
作者：
Srivastava,S;Sasser,G;Driska,SP
本文介绍了一种从猪骨髓中提取肌球蛋白的简便方法。该过程产生了没有任何明显污染物的高纯度肌球蛋白，如原肌球蛋白和肌动蛋白。通过使用几种蛋白酶抑制剂和减少纯化时间，在早期程序中经常观察到的重链蛋白水解大大减少。因此，该前体可用于分离和纯化来自猪盲肠的肌球蛋白，用于高度期望重链的纯度和完整性的研究。
A simple method for preparing myosin from porcine aortae is described. The procedure yields a highly pure myosin devoid of any obvious contaminants, such as tropomyosin and actin. The proteolysis of the heavy chain, frequently observed in the earlier procedures, is greatly minimized by the use of several protease inhibitors and by reducing the purification time. The precedure therefore can be used to isolate and purify myosin from porcine aortae for studies where the purity and integrity of the heavy chain are highly desirable.