Structural organization of the bovine cathelicidin gene family and identification of a novel member
Structural organization of the bovine cathelicidin gene family and identification of a novel member
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DOI：
10.1016/s0014-5793(97)01310-0
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发表时间：
1997-11-17
期刊：
影响因子：
3.5
通讯作者：
Zanetti, M
中科院分区：
文献类型：
--
作者：
Scocchi, M;Wang, SL;Zanetti, M
Cathelicidins 是在多种哺乳动物物种中发现的一组骨髓抗菌肽前体，该家族的转录物显示出高度保守的 5' 区域，对应于 5' 非翻译区、信号肽和前体，以及编码结构不同的 C 端序列的不同 3' 区域，这些序列对应于从前体蛋白水解释放后的成熟抗菌肽。为了确定编码这些蛋白质的牛基因家族的大小，通过筛选利用基于牛成员保守 cDNA 区域的探针构建牛文库，限制性作图、杂交研究和序列分析鉴定了 11 个不同的基因，这些基因基于相邻基因的基因间距离，这些基因似乎物理上非常接近。其中，编码假定的 α 螺旋抗菌肽前体的新基因被识别并测序。该新基因似乎在牛骨髓髓细胞、脾脏和睾丸中表达。 (C) 1997 年欧洲生化学会联合会。
Cathelicidins are a group of myeloid antimicrobial peptide precursors found in a variety of mammalian species, Transcripts of this family show a highly conserved 5' region corresponding to the 5' untranslated region, signal peptide and propiece, and diverse 3' regions encoding structurally varied C-terminal sequences that correspond to mature antimicrobial peptides after proteolytic release from the precursors, To establish the size of the bovine gene family encoding these proteins, lambda genomic clones were isolated by screening a bovine library with a probe based on the conserved cDNA region of bovine members, Restriction mapping, hybridization studies and sequence analysis identified 11 distinct genes that based on the intergenic distances of contiguous genes appear to be in close physical proximity, Among these, a novel gene encoding the precursor of a putative a-helical antimicrobial peptide was recognized and sequenced, The novel gene appears to be expressed in bovine bone marrow myeloid cells, spleen and testis. (C) 1997 Federation of European Biochemical Societies.