Establishment of a mass screening method of sand fly vectors for Leishmania infection by molecular biological methods
Establishment of a mass screening method of sand fly vectors for Leishmania infection by molecular biological methods
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DOI：
10.4269/ajtmh.2007.77.324
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发表时间：
2007-08-01
影响因子：
3.3
通讯作者：
Hashiguchi, Yoshihisa
中科院分区：
文献类型：
--
作者：
Kato, Hirotomo;Uezato, Hiroshi;Hashiguchi, Yoshihisa
监测利什曼原虫及其病媒沙蝇在流行地区和周边地区的流行情况，对于预测利什曼病的风险和扩大具有重要意义。本研究建立了一种大规模筛选沙蝇感染利什曼原虫的方法。野外捕获标本319份，检出阳性沙蝇5只。采用18S rRNA基因聚合酶链反应(PCR) -限制性片段长度多态性（RFLP）鉴定沙蝇种类，阳性蝇均为Lu。hartmanni。此外，细胞色素b （Cyt b）基因序列分析确定所有寄生虫为锥虫内种，包括一个可能的新种。由于该方法需要最少的努力，并且可以一次处理大量样本，因此它将成为研究利什曼病流行病学的有力工具。
Surveillance of the prevalence of Leishmania and its vector, sand fly species, in endemic and surrounding areas is important for prediction of the risk and expansion of leishmaniasis. In this study, a method for the mass screening of sand flies for Leishmania infection was established. This method was applied to 319 field-captured specimens, and 5 positive sand flies were detected. Sand fly species were identified by polymerase chain reaction (PCR) -restriction fragment length polymorphism (RFLP) of the 18S rRNA gene, and all the positive flies were Lu. hartmanni. Furthermore, cytochrome b (Cyt b) gene sequence analyses identified all the parasites as Endotrypanum species including a probable novel species. Because the method requires minimum effort and can process a large number of samples at once, it will be a powerful tool for studying the epidemiology of leishmaniasis.