SPECIFIC-LOCUS TEST SHOWS ETHYLNITROSOUREA TO BE THE MOST POTENT MUTAGEN IN THE MOUSE
SPECIFIC-LOCUS TEST SHOWS ETHYLNITROSOUREA TO BE THE MOST POTENT MUTAGEN IN THE MOUSE
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DOI：
10.1073/pnas.76.11.5818
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
PHIPPS, EL
中科院分区：
文献类型：
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作者：
RUSSELL, WL;KELLY, EM;PHIPPS, EL
使用特定位点试验测量小鼠精原细胞中诱导的传递基因突变频率表明，乙基亚硝基脲是迄今为止在小鼠中发现的最有效的诱变剂。所用剂量为250 mg/kg，产生的突变率是用600 R（最有效的X射线急性剂量）获得的突变率的5倍。最有效剂量的乙基亚硝基脲的致突变性是最有效剂量的甲基苄肼的15倍，甲基苄肼是以前对小鼠最有效的致突变剂。乙基亚硝基脲具有很强的致突变性，可作为研究剂量效应、剂量分级、性别和细胞阶段等因素对化学品致突变作用影响的模型化合物。乙基亚硝基脲显然是在小鼠中产生任何类型的所需新基因突变的诱变剂的选择。
Use of the specific-locus test to measure the frequency of transmitted gene mutations induced in mouse spermatogonia showed that ethylnitrosourea was by far the most potent mutagen yet discovered in the mouse. The dose used, 250 mg/kg, gave a mutation rate 5 times as high as had been obtained with 600 R, the most effective acute dose of X-rays. The most effective dose of ethylnitrosourea was 15 times more mutagenic than the most effective dose of procarbazine, which was previously the most effective mutagen for the mouse. Because of its high mutagenicity, ethylnitrosourea can serve as a model compound in exploring the effect of such factors as dose response, dose fractionation, sex and cell stage on the mutagenic action of a chemical. Ethylnitrosourea is clearly the mutagen of choice for the production of any kind of desired new gene mutations in the mouse.