Indirect ELISA method for detecting the pathogenic bacteria of Litopenaeus vannamei red body disease
Indirect ELISA method for detecting the pathogenic bacteria of Litopenaeus vannamei red body disease
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发表时间：
2006
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通讯作者：
Fan Jing-feng
中科院分区：
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作者：
Fan Jing-feng
已经开发出一种间接酶联免疫吸附测定（ELISA），用于快速诊断副溶血性弧菌（凡纳滨对虾红体病的病原体）。采用棋盘滴定法确定抗原和抗血清的最佳工作浓度分别为106 CFU·mL -1 和1∶2000。测试血清敏感性，最低副溶血弧菌悬浮液为104 CFU。检测抗血清与其他细菌的交叉反应，结果均为阴性。抑制试验中抑制率为75.88%。交叉试验和抑制试验表明该方法具有差异性。优化方法对感染凡纳滨对虾和正常凡纳滨对虾进行检测，阳性检出率分别为93.3%和13.3%。结果表明，该方法不仅可以检测南美白对虾的感染状况，还可以检测携带者。这对于疾病的早期诊断非常重要。
An indirect Enzyme-Linked Immunosorbent Assay (ELISA) for the rapid diagnosis of Vibrio parahaemolyticus, the pathogen of the red body disease of Litopenaeus vannamei, has been developed. The best working concentration of antigen and antiserum were 106 CFU·mL -1 and 1∶2000 separately, determined by using checkerboard titration. The sensitivity of the serum was tested, and the lowest V. parahaemolyticus suspension was 104 CFU. Cross reactions of antisera with other bacteria were detected, all results were negative. Inhibition rate was 75.88% in inhibiton test.The cross assay and the inhibiton assay indicate this method is differential. The method was optimized to detect the infected Litopenaeus vannameiand the normal ones, the positive detecting rate were 93.3% and 13.3% separately. The results indicated that this assay can be used to detect not only the infected unhealthiness of L. vannamei, but also the carriers. This is very important to diagnose the disease in the early stage.