Intestinal epithelial culture under an air-liquid interface: a tool for studying human and mouse esophagi.
Intestinal epithelial culture under an air-liquid interface: a tool for studying human and mouse esophagi.
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气液界面下的肠上皮培养：研究人类和小鼠食管的工具。
DOI：
10.1111/dote.12346
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Mori M.
中科院分区：
文献类型：
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作者：
Yokobori T;Suzuki S;Miyazaki T;Sohda M;Sakai M;Tanaka N;Ozawa D;Hara K;Honjo H;Altan B;Fukuchi M;Ishii H;Iwatsuki M;Sugimachi K;Sudo T;Iwaya T;Nishida N;Mimori K;Kuwano H;Mori M.
本研究调查了肠上皮细胞培养方法是否可应用于小鼠和人食管培养。将从1天大小鼠和成年人身上采集的食道保存在胶原蛋白凝胶中。人食管培养使用市售的人胚胎干细胞培养基。我们发现，肠上皮细胞培养方法可以成功地应用于小鼠和人食管培养。长期培养的食管类器官是由肌成纤维细胞排列的棒状管腔结构。我们发现，食管粘膜表面的再生几乎可以完全在体外进行，这种方法的优点是，可以使用宿主来源的成纤维细胞作为利基产生类器官培养物。这种方法是一种很有前途的工具，小鼠和人类的肠道生物学，致癌作用和再生医学的研究。
This study investigated whether an intestinal epithelial culture method can be applied to mouse and human esophageal cultures. The esophagi harvested from 1-day-old mice and adult humans were maintained in collagen gels. A commercially available culture medium for human embryonic stem cells was used for the human esophageal culture. We discovered that the intestinal epithelial culture method can be successfully applied to both mouse and human esophageal cultures. The long-term cultured esophageal organoids were rod-like luminal structures lined with myofibroblasts. We discovered that regeneration of the esophageal mucosal surface can be almost completely achievedin vitro, and the advantage of this method is that organoid cultures may be generated using host-derived fibroblasts as a niche. This method is a promising tool for mouse and human research in intestinal biology, carcinogenesis, and regenerative medicine.