Characterization of circulatory disorders in beta-thalassemic mice by noninvasive ultrasound biomicroscopy.
Characterization of circulatory disorders in beta-thalassemic mice by noninvasive ultrasound biomicroscopy.
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通过无创超声生物显微镜表征β地中海贫血小鼠的循环障碍。
DOI：
10.1152/physiolgenomics.00305.2005
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发表时间：
2007
影响因子：
4.6
通讯作者：
Cloutier,Guy
中科院分区：
文献类型：
--
作者：
Stoyanova,Ekatherina;Trudel,Marie;Felfly,Hady;Garcia,Damien;Cloutier,Guy
β-地中海贫血是一种遗传性血液病，由β-珠蛋白产生减少或缺乏引起，需要长期治疗干预。这种情况导致重要的动脉和静脉血栓栓塞事件、短暂性脑缺血发作和微循环阻塞，表明循环障碍。为了研究微循环障碍的存在，而没有治疗的混杂效应，我们使用了具有人重型β地中海贫血典型临床特征的β地中海贫血小鼠。理解微循环生理学的一个障碍，特别是对于β-地中海贫血小鼠，一直缺乏适当的非侵入性成像方法。因此，我们开发了一种新的无创高频超声成像方法来评估小鼠血管血流动力学特性。在我们的β-地中海贫血小鼠中，与野生型同窝小鼠相比，总外周血管阻力显著增加（P< 0.01），而平均血压、心率和心输出量相似（P=无显著性）。β-地中海贫血小鼠颈总动脉和腹主动脉的Pourcelot指数均显著降低（P< 0.01和P < 0.05）。因此，我们通过非侵入性超声方法对β-地中海贫血的血管血流动力学进行表征，证明了这些小鼠中微循环流动障碍的存在，并提供了独特的定量评估。
β-Thalassemia is an inherited hematological disease caused by a decrease or absence of production of β-globin that requires chronic therapeutic interventions. This condition leads to important arterial and venous thromboembolic events, transitory ischemic attacks, and microcirculatory obstructions, indicative of circulatory disturbances. To investigate the presence of microcirculatory disorders without the confounding effect of treatments, we used β-thalassemic mice with typical clinical characteristics of human β-thalassemia major. One impediment to the understanding of microcirculatory physiology, in particular for β-thalassemic mice, has been the lack of an appropriate noninvasive imaging approach. We thus developed a novel noninvasive high-frequency ultrasound imaging method to evaluate murine vascular hemodynamic properties. In our β-thalassemic mice, total peripheral vascular resistance was significantly increased (P< 0.01) compared with wildtype littermates, whereas mean blood pressure, heart rate, and cardiac output were similar (P= nonsignificant). Importantly, the vascular hemodynamics in β-thalassemic mice were significantly affected according to the Pourcelot indexes measured in the common carotid artery and abdominal aorta (P< 0.01 andP< 0.05, respectively). Hence, our β-thalassemia characterization of vascular hemodynamics by noninvasive ultrasonic approaches proves the existence and provides unique quantitative assessment of microcirculatory flow disturbances in those mice.