Ultrahigh-resolution NMR spectroscopy.
Ultrahigh-resolution NMR spectroscopy.
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DOI：
10.1002/anie.201404111
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发表时间：
2014-07-01
影响因子：
16.6
通讯作者：
Morris, Gareth A.
中科院分区：
文献类型：
--
作者：
Foroozandeh, Mohammadali;Adams, Ralph W.;Meharry, Nicola J.;Jeannerat, Damien;Nilsson, Mathias;Morris, Gareth A.
光谱分辨率在NMR光谱学中至关重要，但受仪器限制。最近的“纯位移”脉冲序列的发展大大提高了分辨率，但往往在灵敏度的高成本。我们介绍了一类新的纯位移实验（PSYCHE）具有上级灵敏度，光谱纯度，和容忍强耦合。任何光谱技术的关键参数是灵敏度和分辨率。在NMR光谱学的情况下，通过引入傅立叶变换方法[1]和改进的探针技术提供的灵敏度增益使得光谱分辨率成为大多数应用的限制因素。在核磁共振波谱学的早期，人们已经认识到，对于某些原子核，特别是质子，如果能抑制同素核自旋-自旋耦合的影响，就能获得很大的分辨率，但多年来，所有提出的方法都或多或少地证明是不令人满意的。最近，出现了更实用的方法，所谓的“纯位移”或“化学位移”方法，其部分或完全抑制同素偶联的影响，从而产生由每个化学上不同的位点的单个信号组成的光谱，但通常在灵敏度方面代价很高。在这里，我们提出了一种通用的新方法，纯位移NMR光谱，具有约10倍的灵敏度比竞争的方法，提供干净的光谱，并容忍强耦合。
Spectral resolution is vital in NMR spectroscopy, but is instrument-limited. Recent “pure shift” pulse-sequence developments greatly improve resolution, but often at a high cost in sensitivity. We introduce a new class of pure shift experiments (PSYCHE) with superior sensitivity, spectral purity, and tolerance of strong coupling. The key parameters for any spectroscopic technique are sensitivity and resolution. In the case of NMR spectroscopy, the sensitivity gains provided by the introduction of Fourier transform methods [1] and of improved probe technologies make spectral resolution the limiting factor for most applications. From the early days of NMR spectroscopy it has been recognized that for some nuclei, in particular the proton, large gains in resolution could be achieved if the effects of homonuclear spin–spin couplings could be suppressed, but for many years all the methods proposed proved more or less unsatisfactory. Recently, much more practical methods, socalled “pure shift” or “chemical shift” methods have emerged, which partially or completely suppress the effects of homonuclear coupling, thereby generating spectra consisting of a single signal for each chemically distinct site, but generally at a high price in terms of sensitivity. Here we present a versatile new approach to pure shift NMR spectroscopy which has approximately tenfold better sensitivity than competing methods, gives clean spectra, and is tolerant of strong coupling.
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影响因子：
2.2
作者：
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通讯作者：
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