Functional characterization of the S-cerevisiae genome by gene deletion and parallel analysis
Functional characterization of the S-cerevisiae genome by gene deletion and parallel analysis
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DOI：
10.1126/science.285.5429.901
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发表时间：
1999-08-06
期刊：
影响因子：
56.9
通讯作者：
Davis, RW
中科院分区：
文献类型：
--
作者：
Winzeler, EA;Shoemaker, DD;Davis, RW
在基因组测序项目中鉴定出的许多开放阅读框（ORF）的功能是未知的。需要新的全基因组方法来系统地确定它们的功能。通过一种高通量策略构建了总共6925株酿酒酵母菌株，每个菌株都精确缺失了2026个ORF中的一个（超过基因组中ORF的三分之一）。在缺失的ORF中，17%对于在丰富培养基中的生存是必需的。对500多个缺失菌株的表型进行了平行检测。在这些缺失菌株中，40%在丰富培养基或基本培养基中显示出定量的生长缺陷。
The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.