Open systems: panoramic views of gene expression
Open systems: panoramic views of gene expression
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DOI：
10.1016/s0022-1759(01)00306-4
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发表时间：
2001-04-01
影响因子：
2.2
通讯作者：
Lewin, DA
中科院分区：
文献类型：
--
作者：
Green, CD;Simons, JF;Lewin, DA
自20世纪90年代初发展以来，差异基因表达(DGE)技术已被应用于许多生物学挑战，既用于基础生物学研究，也作为发现和开发药物的宝贵工具。在这篇综述中，我们概述了一类统称为“开放”体系结构系统的DGE技术。这些技术与封闭的DGE技术(定量聚合酶链式反应、芯片技术)的不同之处在于，不需要预先存在的生物或序列信息，它们适用于任何物种。开放系统的例子包括GeneCling(R)：、SAGE、TOGA、READS(TM)及其前身DGE技术、差异显示和cDNA代表性差异分析。我们回顾了这些技术，并总结了使用GeneCall进行新基因发现的具体应用。此外，还讨论了数据管理和实验设计在这个表达分析的新时代的意义。(C)2001年爱思唯尔科学公司。版权所有。
Since their development in the early 1990s, differential gene expression (DGE) technologies have been applied to a multitude of biological challenges, both for the purpose of basic biological research and as a valuable tool for the discovery and development of pharmaceuticals. In this review we survey a class of DGE technologies collectively referred to as 'open' architecture systems. These technologies are distinct from the 'closed' DGE technologies (quantitative PCR, chip technologies), in that no pre-existing biological or sequence information is necessary and they are applicable to any species. Examples of open systems include GeneCalling (R):, SAGE, TOGA, READS (TM) and their progenitor DGE technologies, differential display and cDNA representational difference analysis. We review these technologies and summarize a specific application using GeneCalling for novel gene discovery. Additionally, the significance of data management and experimental design in this new age of expression analysis is discussed. (C) 2001 Elsevier Science BN. All rights reserved.