Pathways in bacterial and archaeal communities dictated by ammonium stress in a high solid anaerobic digester with dewatered sludge
Pathways in bacterial and archaeal communities dictated by ammonium stress in a high solid anaerobic digester with dewatered sludge
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脱水污泥高固体厌氧消化池中氨胁迫决定的细菌和古菌群落的途径
DOI：
10.1016/j.biortech.2017.05.094
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发表时间：
2017
影响因子：
11.4
通讯作者：
Dai Xiaohu
中科院分区：
文献类型：
--
作者：
Li Ning;He Jin;Yan Han;Chen Sisi;Dai Xiaohu
进行微生物谱的宏基因组学比较，以研究在污水污泥的高固体厌氧消化器的110天操作期间来自稳定（第42天）、铵调节（第63天）和铵应激（第102天）时期的样品之间的差异。与稳定期相比，氨调节后沼气产量略有下降，在22个氨相关基因中，有6个基因的丰度较高。此外，在19个氨基酸相关基因中，有9个与氨基酸生成和利用有关的基因表达量降低，这部分揭示了铵胁迫后挥发性固体（VS）降解恶化的原因。此外，虽然乙酸裂解途径在一定程度上抑制与沼气量和内容的减少，没有增强参与氢营养产甲烷的基因被观察到，阐明了不同的作用，铵胁迫指导细菌群落结构朝着增强互养乙酸氧化反应。
Metagenomic comparisons of microbial profiles were conducted to investigate differences between the samples from steady (Day 42), ammonium-adjusting (Day 63), and ammonium-stressed (Day 102) periods during the 110-day operation of a high solid anaerobic digester of sewage sludge. Comparing to the steady period, biogas production was slightly inhibited after ammonium adjustment, during which the microbes showed higher abundance in 6 of the total 22 ammonium-related genes. In addition, among the 19 amino-acid-related genes, 9 genes involved in amino acid generation and utilization were reduced, which partially revealed the reason of deterioration of volatile solids (VSs) degradation following ammonium stress. Furthermore, although the acetoclastic pathway was to some extent inhibited with the decrease of biogas amount and content, no enhancement of genes involved in hydrogenotrophic methanogenesis was observed, elucidating the distinct role of ammonium stress in directing bacterial community structure toward the enhanced syntrophic acetate oxidation reaction.