Identification and profiling of sex-biased microRNAs from sea urchin Strongylocentrotus nudus gonad by Solexa deep sequencing
Identification and profiling of sex-biased microRNAs from sea urchin Strongylocentrotus nudus gonad by Solexa deep sequencing
复制标题
通过 Solexa 深度测序对海胆 Strongylocentrotus nudus 性腺中性别偏向的 microRNA 进行鉴定和分析
DOI：
10.1016/j.cbd.2014.01.001
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发表时间：
2014-06-01
影响因子：
3
通讯作者：
Liu, Xiaolin
中科院分区：
文献类型：
--
作者：
Mi, Xiao;Wei, Zhenlin;Liu, Xiaolin
MicroRNAs （miRNAs）是一类内源性小的非编码RNA，通过转录后抑制信使RNA来调节基因表达。棘皮动物圆心圆齿（Strongylocentrotus nudus）是一种极好的研究发育的模式生物，也是一种重要的商业食物来源。然而，到目前为止，还没有microrna被报道调节裸鼠性腺分化。在这项研究中，我们分别构建了雄性和雌性裸裸雌s性腺的两个小RNA文库，用于Solexa测序。从两个文库中共鉴定出184个mirna，包括60个已知mirna和124个新mirna。此外，还分析了已知mirna的核苷酸偏差和末端变异。此外，通过比较两个文库中的miRNA表达谱，发现86个共表达miRNA和98个性别特异性miRNA（47个男性特异性和51个女性特异性）中67个表达不同，这些miRNA可能参与性别分化。本研究首次揭示了裸鼠性腺分化相关的miRNA图谱。该研究首次揭示了海胆miRNA表达的性别差异，为miRNA在生殖器官特异性作用的研究提供了基础。(C) 2014爱思唯尔公司版权所有。
MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs that regulate gene expression by post-transcriptional repression of messenger RNA. The echinoderm, Strongylocentrotus nudus, is an excellent model organism for studying development and commercially important as a food source. However, to date no miRNAs have been reported to modulate sex gonad differentiation in S. nudus. In this study, we constructed two small RNA libraries from male and female S. nudus gonad respectively for Solexa sequencing. A total of 184 miRNAs including 60 known and 124 novel miRNAs were identified from the two libraries. Furthermore, the nucleotide bias and end variation of the known miRNAs were also analyzed. In addition, 67 differently expressed of the 86 co-expressed and 98 gender-specific (47 male-specific and 51 female-specific) miRNAs that may be involved in sexual differentiation were found by comparing the miRNA expression profiles in the two libraries. This study reveals the first miRNA profile related to the gonad differentiation of the S. nudus. This study gives a first insight into sex differences in miRNA expression of sea urchin which could facilitate studies of the reproductive organ-specific roles of miRNAs. (C) 2014 Elsevier Inc. All rights reserved.