Contaminated liquid nitrogen vapour as a risk factor in pathogen transfer
Contaminated liquid nitrogen vapour as a risk factor in pathogen transfer
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DOI：
10.1016/j.theriogenology.2008.12.011
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发表时间：
2009-04-15
期刊：
影响因子：
2.8
通讯作者：
Morris, G. J.
中科院分区：
文献类型：
--
作者：
Grout, B. W. W.;Morris, G. J.
储存容器中的液氮会从大气或放置在其中的容器表面收集颗粒物。随着时间的推移。其中一些积聚的颗粒可能是致病生物，可以证明，浸泡在液氮中可以保存它们的生存能力。这种污染对储存样品的状态构成风险，可以。在很大程度上，可以通过使用适当的技术来清除样品容器并对其外表面进行消毒来避免。目前的研究使用真菌孢子和有机晶体来证明，当氮气蒸汽冷却可编程冷冻机或干货轮时，液氮中含有的这些颗粒被释放回环境中。这表明，在液氮上方的气相中存储仍然存在样品或设备污染的实际风险。无论储存的样品是否安全，这都是本地和国际上储存库或实验地点之间交叉污染的潜在来源。这可能对临床和农业造成严重后果。这项研究为这种可能性提供了证据。由于无法量化，应纳入生殖材料储存方案的风险分析。当。例如，精液和胚胎在一个农业场所冷冻，干运输商可以将污染农作物病原体的孢子共同运输到目的地。(c) 2009爱思唯尔公司版权所有。
Liquid nitrogen in storage containers will gather particulate matter From the atmosphere, or the surfaces of containers placed into it. with time. Some of these accumulating particles may be pathogenic organisms and it can be demonstrated that their viability may be conserved by immersion in the liquid nitrogen. This contamination constitutes a risk to the status of stored samples that can. largely, be avoided by the use of appropriate techniques for scaling sample containers and sterilizing their outer surfaces. The present study uses fungal spores and organic crystals to demonstrate that such particles contained in liquid nitrogen are released back into the environment when nitrogen vapour cools programmable freezers or dry shippers. This demonstrates that storage in the vapour phase above liquid nitrogen still carries a real risk of sample, or facility, contamination. Regardless of the safety of the stored sample, this is a potential source of cross-contamination between repositories or experimental sites, both locally and internationally. that could have serious consequences in clinical and agricultural situations. This study provides evidence 10 suggest that this possibility. as vet unquantified, should be included in risk analysis of storage protocols for reproductive materials. The risk becomes diverse when. for example, semen and embryos are frozen at an agricultural site and the dry shipper can co-transport spores of contaminating crop plant pathogens to the destination site. (c) 2009 Elsevier Inc. All rights reserved.