Schisandrin B induces apoptosis and cell cycle arrest of gallbladder cancer cells.
Schisandrin B induces apoptosis and cell cycle arrest of gallbladder cancer cells.
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DOI：
10.3390/molecules190913235
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发表时间：
2014-08-27
期刊：
影响因子：
--
通讯作者：
Liu YB
中科院分区：
文献类型：
--
作者：
Xiang SS;Wang XA;Li HF;Shu YJ;Bao RF;Zhang F;Cao Y;Ye YY;Weng H;Wu WG;Mu JS;Wu XS;Li ML;Hu YP;Jiang L;Tan ZJ;Lu W;Liu F;Liu YB
胆囊癌是胆管最常见的恶性肿瘤，侵袭性高，预后极差。该论文的主要目的是研究五味子 B (Sch B) 对胆囊癌细胞的影响，并确定其潜在抗癌作用的机制。我们通过 MTT 和集落形成测定表明 Sch B 以剂量、时间依赖性方式抑制人胆囊癌细胞的活力和增殖，并通过流式细胞术以剂量依赖性方式降低线粒体膜电位 (ΔΨm)。流式细胞术分析还揭示了 GBC-SD 和 NOZ 细胞的 G0/G1 期停滞和细胞凋亡。 Sch B 处理细胞的蛋白质印迹分析显示 Bax、cleaved caspase-9、cleaved caspase-3、cleaved PARP 上调，Bcl-2、NF-κB、细胞周期蛋白 D1 和 CDK-4 下调。此外，该药物还抑制皮下NOZ肿瘤异种移植裸鼠的肿瘤生长。这些数据表明Sch B通过调节凋亡相关蛋白的表达来诱导胆囊癌细胞凋亡，并表明Sch B可能是治疗胆囊癌的有前途的药物。
Gallbladder cancer, with high aggressivity and extremely poor prognosis, is the most common malignancy of the bile duct. The main objective of the paper was to investigate the effects of schisandrin B (Sch B) on gallbladder cancer cells and identify the mechanisms underlying its potential anticancer effects. We showed that Sch B inhibited the viability and proliferation of human gallbladder cancer cells in a dose-, time -dependent manner through MTT and colony formation assays, and decrease mitochondrial membrane potential (ΔΨm) at a dose-dependent manner through flow cytometry. Flow cytometry assays also revealed G0/G1 phase arrest and apoptosis in GBC-SD and NOZ cells. Western blot analysis of Sch B-treated cells revealed the upregulation of Bax, cleaved caspase-9, cleaved caspase-3, cleaved PARP and downregulation of Bcl-2, NF-κB, cyclin D1 and CDK-4. Moreover, this drug also inhibited the tumor growth in nude mice carrying subcutaneous NOZ tumor xenografts. These data demonstrated that Sch B induced apoptosis in gallbladder cancer cells by regulating apoptosis-related protein expression, and suggests that Sch B may be a promising drug for the treatment of gallbladder cancer.
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发表时间：
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影响因子：
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影响因子：
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作者：
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