PURIFICATION AND PROPERTIES OF ACID-PHOSPHATASE SECRETED FROM LUPIN ROOTS UNDER PHOSPHORUS-DEFICIENCY CONDITIONS
PURIFICATION AND PROPERTIES OF ACID-PHOSPHATASE SECRETED FROM LUPIN ROOTS UNDER PHOSPHORUS-DEFICIENCY CONDITIONS
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DOI：
10.1080/00380768.1995.10419608
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发表时间：
1995-09-01
影响因子：
2
通讯作者：
TADANO, T
中科院分区：
文献类型：
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作者：
OZAWA, K;OSAKI, M;TADANO, T
在缺磷营养液中，羽扇豆根系分泌的酸性磷酸酶活性显著高于磷充足条件下的活性。酸性磷酸酶的IEF染色结果表明，在根和叶中有多种同工酶，但有一种主要分泌到根际。从羽扇豆生长的缺磷营养液中，用DEAE-Sepharose CL-6 B离子交换层析、Bio-Gel P-200凝胶过滤和电泳分离纯化了这种从根部分泌的主要酸性磷酸酶。纯化的酶的分子量通过SDS-PAGE估计为72，000，通过凝胶过滤估计为约140，000。因此，该酶被认为是同源二聚体。该酶的等电点为4.7，最适pH为4.3。
When lupin plants were grown in phosphorus-deficient nutrient solutions, the activity of acid phosphatase secreted from their roots remarkably increased in comparison with that under adequate phosphorus conditions. Based on activity staining of acid phosphatase on IEF gels, many isozymes mere present in roots and leaves, but one of these was mainly secreted into the rhizosphere. This main acid phosphatase secreted from roots was purified from the phosphorus-deficient nutrient solution where lupin was grown, using ion exchange chromatography with DEAE-Sepharose CL-6B, gel filtration with Bio-Gel P-200, and preparative-PAGE. The molecular weight of the purified enzyme was estimated to be 72,000 by SDS-PAGE and approximately 140,000 by gel filtration. Thus, this enzyme was considered to be a homo-dimer. The isoelectric point and the optimum pH of the enzyme were 4.7 and 4.3, respectively.