Electrostatically promoted dynamic hybridization of glucans with cationic polythiophene
Electrostatically promoted dynamic hybridization of glucans with cationic polythiophene
复制标题
静电促进葡聚糖与阳离子聚噻吩的动态杂交
DOI：
10.1039/c6ob01353h
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发表时间：
2016
影响因子：
3.2
通讯作者：
Inoue Yoshihisa
中科院分区：
文献类型：
--
作者：
Fukuhara Gaku;Imai Mami;Fuentealba Denis;Ishida Yuki;Kurohara Hiroki;Yang Cheng;Mori Tadashi;Uyama Hiroshi;Bohne Cornelia;Inoue Yoshihisa
天然高分子与合成高分子的杂化是构建复杂超分子结构的有效方法。为了全面阐明葡聚糖与合成聚合物的杂交机理，采用紫外-可见光谱、圆二色谱、荧光光谱、荧光激发光谱、核磁共振光谱等方法，结合理论计算、动态光散射、红外光谱、紫外-可见光谱、红外光谱、核磁共振等方法，研究了三链凝胶多糖（Cur）和三链脱乙酰葡聚糖（SPG）与阳离子聚噻吩（PyPT）在DMSO水溶液中的杂交行为，和ζ电位测量。在与葡聚糖混合时，形成异源三链体，其是动态的并且通过加热和通过添加碱或盐而大大加速。异源三链体在高碱性溶液中分解成异源双链体。因此，聚阳离子聚合物，如PyPT，预计将作为一个通用的工具，解压缩葡聚糖同源三链体，并促进随后的杂交在水溶液中，而详细的机制阐明在本研究中有助于杂交合作伙伴的合理设计。
Hybridizing natural macromolecules with synthetic polymers is an efficient general method for constructing sophisticated supramolecular architectures. To comprehensively elucidate the controversial hybridization mechanism of glucans with synthetic polymers, the hybridization behaviors of triple-stranded curdlan (Cur) and schizophyllan (SPG) with cationic polythiophene (PyPT) were investigated in aqueous DMSO solutions by using UV-vis, circular dichroism (CD), fluorescence, fluorescence excitation, and NMR spectroscopy methods, as well as theoretical calculations, dynamic light scattering, and zeta potential measurements. Upon mixing with glucan, a hetero-triplex formed, which was dynamic and greatly accelerated by heating and by adding a base or a salt. The hetero-triplex disassembled into a hetero-duplex in highly basic solutions. Thus, polycationic polymers, such as PyPT, are expected to serve as a versatile tool for unzipping glucan homo-triplexes and promoting subsequent hybridization in aqueous solution, while the detailed mechanism elucidated in the present study contributes to the rational design of hybridization partners.