Effects of Early Intraoral Acesulfame-K Stimulation to Mice on the Adults Sweet Preference and the Expression of -Gustducin in Fungiform Papilla
Effects of Early Intraoral Acesulfame-K Stimulation to Mice on the Adults Sweet Preference and the Expression of -Gustducin in Fungiform Papilla
复制标题
早期口内安赛蜜刺激小鼠对成年甜味偏好及菌状乳头β-味导素表达的影响
DOI：
10.1093/chemse/bjt001
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发表时间：
2013-06-01
期刊：
影响因子：
3.5
通讯作者：
Deng, Shao-Ping
中科院分区：
文献类型：
--
作者：
Chen, Meng-Ling;Liu, Si-Si;Deng, Shao-Ping
人工甜味剂乙酰磺胺钾（AK）在早期发育阶段暴露可能会影响成年人的甜味偏好和周边味觉系统。我们观察到，从出生后第4天（P4）到断奶的小鼠口内AK刺激降低了成年期对AK和蔗糖溶液的偏好阈值，而偏好模式不变。与对照组相比，在一定浓度范围内的AK或蔗糖溶液暴露组的偏好评分显着增加。同时，更多的-Gustducin标记的菌状味蕾和细胞在一个单一的味蕾诱导从第7周的早期口内AK刺激。然而，增长的数量-Gustducin阳性味蕾或阳性细胞数每味蕾只发生在前面的区域，喙1毫米的部分，但不是在中间区域，尾部4毫米的部分，前三分之二的舌头含有菌状乳头。这项工作扩展了我们以前的观察，并提供了新的信息-Gustducin在小鼠菌状味蕾早期AK刺激条件下的发展和区域表达模式。
Exposure to artificial sweetener acesulfame-K (AK) at early development stages may influence the adult sweet preference and the periphery gustatory system. We observed that the intraoral AK stimulation to mice from postnatal day 4 (P4) to weaning decreased the preference thresholds for AK and sucrose solutions in adulthood, with the preference pattern unchanged. The preference scores were increased in the exposure group significantly when compared with the control group at a range of concentrations for AK or sucrose solution. Meanwhile, more -Gustducin-labeled fungiform taste buds and cells in a single taste bud were induced from week 7 by the early intraoral AK stimulation. However, the growth in the number of -Gustducin-positive taste bud or positive cell number per taste bud occurred only in the anterior region, the rostral 1-mm part, but not in the intermediate region, the caudal 4-mm part, of the anterior two-third of the tongue containing fungiform papillae. This work extends our previous observations and provides new information about the developmental and regional expression pattern of -Gustducin in mouse fungiform taste bud under early AK-stimulated conditions.