Early expression of Tubulin Beta-III in avian cranial neural crest cells
Early expression of Tubulin Beta-III in avian cranial neural crest cells
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DOI：
10.1016/j.gep.2019.119067
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发表时间：
2019-12-01
影响因子：
1.2
通讯作者：
Rogers, Crystal D.
中科院分区：
文献类型：
--
作者：
Chacon, Jose;Rogers, Crystal D.
神经嵴细胞是一种短暂的干细胞样细胞群，其形成于脊椎动物胚胎的背神经管中，然后迁移到不同的位置以分化成不同的衍生物，如颅面骨、软骨以及肠和外周神经系统。目前的神经嵴细胞发育的教条表明，有一个特定的分层基因调控网络（GRN），控制这些细胞在特定的发育时间的诱导，规范和分化。我们的实验室已经确定，分化神经元的标志物微管蛋白β-III（TUBB 3）在迁移前神经嵴细胞中表达。TUBB 3以前已被确定为微管的主要组成部分，并在发育过程中为轴突的正确引导和维护所需。使用的模式生物，原鸡，我们的特点是时空定位的TUBB 3在发展的早期阶段。在这里，我们显示TUBB 3在发育中的神经板中表达，在细胞分层和迁移之前在迁移前的颅神经嵴中上调，并且在后期发育阶段的神经元中维持或上调。我们认为TUBB 3可能在早期神经嵴形成和迁移中起作用，而不是在神经发生中起作用。
Neural crest cells are a transient stem-like cell population that forms in the dorsal neural tube of vertebrate embryos and then migrates to various locations to differentiate into diverse derivatives such as craniofacial bone, cartilage, and the enteric and peripheral nervous systems. The current dogma of neural crest cell development suggests that there is a specific hierarchical gene regulatory network (GRN) that controls the induction, specification, and differentiation of these cells at specific developmental times. Our lab has identified that a marker of differentiated neurons, Tubulin Beta-III (TUBB3), is expressed in premigratory neural crest cells. TUBB3 has previously been identified as a major constituent of microtubules and is required for the proper guidance and maintenance of axons during development. Using the model organism, Gallus gallus, we have characterized the spatiotemporal localization of TUBB3 in early stages of development. Here we show TUBB3 is expressed in the developing neural plate, is upregulated in the pre-migratory cranial neural crest prior to cell delamination and migration, and it is maintained or upregulated in neurons in later developmental stages. We believe that TUBB3 likely has a role in early neural crest formation and migration separate from its role in neurogenesis.