SV40 early mutants that are defective for viral DNA synthesis but competent for transformation of cultured rat and simian cells.
SV40 early mutants that are defective for viral DNA synthesis but competent for transformation of cultured rat and simian cells.
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SV40 早期突变体，病毒 DNA 合成有缺陷，但能够转化培养的大鼠和猿细胞。
DOI：
10.1016/0042-6822(82)90296-3
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发表时间：
1982
期刊：
影响因子：
3.7
通讯作者：
Ahrens,B
中科院分区：
文献类型：
--
作者：
Gluzman,Y;Ahrens,B
通过回收整合到C6细胞基因组中的病毒DNA，获得了一株早期的SV40突变体(C6/SV40)。C6/SV40突变体的DNA不能在允许的猿猴细胞中复制，但它能高效地转化它们。它还可以转化已建立的大鼠细胞，其效率与野生型DNA相同，尽管突变转化细胞的表型与野生型转化子的表型略有不同。基因分析表明，C6/SV40携带多种突变。这些突变被部分分离到单独的病毒基因组C6-1和C6-2中。核苷酸序列分析表明，C6-1和C6-2的突变均为颠换(C6-1中5074位核苷酸G到T，5011位A到T，C6-2中4360位核苷酸A到C)，所有的核苷酸都在SV系统中(J.Tooze(1982)，《肿瘤病毒的分子生物学》，第2部分，《DNA肿瘤病毒》，冷泉港实验室，冷泉港，纽约)。预测的氨基酸变化是C6-1的Met-Ile和Lys-Asn，C6-2的Asn-Thr。在C6-1的情况下，这些替换发生在与小T抗原(氨基酸30和51)共同持有的大T抗原的N端部分。C6-2中突变的氨基酸位于大T抗原第153位的独特部分。C6-1和C6-2都像野生型SV40一样有效地转化已建立的大鼠细胞。然而，在允许的CV1细胞中没有检测到C6-2DNA的复制，而C6-1DNA的复制发生时效率显著降低。分离到C6-1的一个回复突变体，发现保留了两个原始突变，并获得了一个新的突变(第4977位G到A)，预测了Glu-Lys在63位氨基酸的氨基酸变化，这在大T抗原和小T抗原中都是常见的。
An early SV40 mutant (C6/SV40) was isolated by rescuing the viral DNA integrated into the genome of the C6 cell line which is a CV1 cell line transformed with uv-irradiated SV40. The DNA of C6/SV40 mutant does not replicate detectably in permissive, simian cells, but it transforms them with high efficiency. It also transforms established rat cells with an efficiency equal to that of wild-type DNA, although the phenotype of the mutant-transformed cells is somewhat different from that of the wild-type transformants. Genetic analysis shows that C6/SV40 carries multiple mutations. These mutations were partially segregated into separate viral genomes, C6-1 and C6-2. Nucleotide sequencing showed that the mutations in both C6-1 and C6-2 were transversions (G to T at nucleotide 5074 and A to T at nucleotide 5011 in C6-1, and A to C at nucleotide 4360 in C6-2); all nucleotide numbers are in SV system (J. Tooze (ed.) (1982), “Molecular Biology of Tumor Viruses,” Part 2, “DNA Tumor Viruses,” Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.). The predicted amino acid changes are Met-Ile and Lys-Asn in C6-1 and Asn-Thr in C6-2. In the case of C6-1, these substitutions would occur in the N-terminal portion of large T antigen that is held in common with small t antigen (amino acids 30 and 51). The mutated amino acid in C6-2 resides in the unique portion of large T antigen at position 153. Both C6-1 and C6-2 transform established rat cells as efficiently as wild-type SV40. However, no replication of C6-2 DNA is detectable in permissive CV1 cells, while replication of C6-1 DNA occurs with significantly reduced efficiency. A revertant of C6-1 was isolated and found to have retained the two original mutations and to have acquired a new mutation (G to A at nucleotide 4977), predicting an amino acid change of Glu-Lys at amino acid position 63, which is common to both large and small T antigens.
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细菌中动物病毒突变体的分离。
影响因子：
56.9
作者：
K. Peden;J. Pipas;S. Pearson;D. Nathans
通讯作者：
D. Nathans
T 抗原相关蛋白在 SV40 DNA 上的结合位点
影响因子：
64.5
作者：
R. Tjian
通讯作者：
R. Tjian
猿猴病毒40的tsA突变体转化BALB/c-3T3细胞：转化表型的温度敏感性和野生型病毒的再转化
影响因子：
5.4
作者：
W. W. Brockman
通讯作者：
W. W. Brockman
源自质粒 ColE1、F、R6K 和 RK2 的质粒克隆载体。
影响因子：
--
作者：
M. Kahn;R. Kolter;C. Thomas;D. Figurski;R. Meyer;E. Remaut;D. Helinski
通讯作者：
D. Helinski
T抗原对猿猴病毒40基因A的自动调节。
DOI：
10.1073/pnas.73.9.3083
发表时间：
1976
影响因子：
11.1
作者：
S. Reed;G. Stark;J. Alwine
通讯作者：
J. Alwine