Characterization and phylogenetic implications of the complete mitochondrial genome of Idiocerinae (Hemiptera: Cicadellidae)
Characterization and phylogenetic implications of the complete mitochondrial genome of Idiocerinae (Hemiptera: Cicadellidae)
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Idiocerinae（半翅目：蝉科）完整线粒体基因组的特征和系统发育意义
DOI：
10.1016/j.ijbiomac.2018.08.191
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发表时间：
2018-12-01
影响因子：
8.2
通讯作者：
Wang, Xian-Yi
中科院分区：
文献类型：
--
作者：
Wang, Jia-Jia;Yang, Mao-Fa;Wang, Xian-Yi
在本研究中，我们测定并分析了4种棘虫科（Liocratus salicis, idiscopus myrica， populicus populi和Parocerus laurifoliae）（半翅目：棘虫科）的线粒体全基因组（mitogenome）。Idiocerinae有丝分裂基因组大小从15,287 bp （idiocerus nitidulus）到16,811 bp （Parocerus laurifoliae）不等。有丝分裂基因组在基因含量、基因大小、基因顺序、碱基组成、蛋白质编码基因密码子使用和二级RNA结构等方面与其他叶蝉相似，具有高度的保守性。大多数蛋白质编码基因以ATN开始，以TAA或TAG结束。16S RNA的二级结构包含6个结构域，42个螺旋；12S RNA的二级结构域包含3个结构域，24个螺旋。此外，在Liocratus固体中观察到tRNA-Ile （AAT）的多拷贝（76 bp），位于对照区和tRNA-l-tRNA-Q-tRNA-M之间。系统发育分析表明，在蝉科中，每个亚科都是单系的，三角头叶蝉亚科构成一个林中林，并倾向于作为其他叶蝉亚科的姐妹类群被置于树的基部。大眼蝇科、粗尾蝇科和跳树蝇科的群落形成了一个具有较高支撑力的林间空地。(C) 2018 Elsevier B.V.版权所有
In this study, we determined and analyzed the complete mitochondrial genomes (mitogenomes) of four species of Idiocerinae (Liocratus salicis, Idioscopus myrica, Populicerus populi and Parocerus laurifoliae) (Hemiptera: Cicadellidae). The Idiocerinae mitogenomes ranged in size from 15,287 (Idioscopus nitidulus) to 16,811 bp (Parocerus laurifoliae). The mitogenomes were highly conserved regarding gene content, gene size, gene order, base composition, codon usage of protein-coding genes, and secondary RNA structures, which were similar to those of other leafhoppers. Most protein-coding genes began with ATN and ended with either TAA or TAG. The secondary structure of the 16S RNA contained six domains with 42 helices, and the 12S RNA comprised three domains with 24 helices. Additionally, a multicopy of tRNA-Ile (AAT) (76 bp) was observed in Liocratus solids, located between the control region and tRNA-l-tRNA-Q-tRNA-M. Phylogenetic analyses showed that each subfamily was recovered as monophyletic within Cicadellidae, and Deltocephalinae species constituted one Glade and tended to be placed at the basal position of the tree as the sister group to the other leafhopper subfamilies. The Megophthalminae, Idiocerinae, and the treehoppers always formed a Glade with high support values. (C) 2018 Elsevier B.V. All rights reserved.