Microtubule polarity confers direction to pigment transport in chromatophores.
Microtubule polarity confers direction to pigment transport in chromatophores.
复制标题
微管极性赋予色素在色素细胞中运输的方向。
DOI：
10.1083/jcb.103.4.1547
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Porter,KR
中科院分区：
文献类型：
--
作者：
McNiven,MA;Porter,KR
人们对用于指导细胞器易位的细胞机制知之甚少。据信，微管的内在结构极性可能在此过程中发挥作用。我们研究了不同取向的微管对手术切断的黑素细胞臂中色素运输方向的影响。在之前的一篇论文（McNiven, M. A., M. Wang, 和 K. R. Porter, 1984, Cell, 37:753-765）中，我们报道了分离后，手臂重新极化并重新定向其微管从中心向外，仿佛形成新的“微细胞”。这些手臂中的色素聚集朝向位于手臂中心的新焦点。在这项研究中，我们监测了含有紫杉醇稳定微管的孤立臂中的色素运动，以测试色素运输方向的逆转是否依赖于微管的复极化。我们报告说，紫杉醇以浓度依赖性方式延迟微管重新定向和运输方向逆转。这些和其他提供的数据表明，黑素细胞内微管群的极性决定了色素运输的方向。
The cellular mechanisms used to direct translocating organelles are poorly understood. It is believed that the intrinsic structural polarity of microtubules may play a role in this process. We have examined the effects that differently oriented microtubules have upon the direction of pigment transport in surgically severed melanophore arms. In a previous paper (McNiven, M. A., M. Wang, and K. R. Porter, 1984, Cell, 37:753-765) we reported that after isolation, arms repolarized and reoriented their microtubules outward from their centers as if to form new "microcells." Pigment aggregation in these arms was toward a new focal point located at the arm centers. In this study we monitored pigment movement in isolated arms containing taxol-stabilized microtubules to test if the reversal in direction of pigment transport is dependent upon the repolarization of microtubules. We report that taxol delays both the microtubule reorientation and reversal in transport direction in a concentration-dependent manner. These and other presented data suggest that the polarity of the microtubule population within a melanophore confers direction on pigment transport.