Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species.
Interspecies reactivities of anti-human macrophage monoclonal antibodies to various animal species.
复制标题
抗人巨噬细胞单克隆抗体对各种动物物种的种间反应。
DOI：
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发表时间：
1996
影响因子：
3.2
通讯作者：
K. Takahashi
中科院分区：
文献类型：
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作者：
L. Zeng;M. Takeya;X. Ling;A. Nagasaki;K. Takahashi
我们研究了八种抗人单核细胞/巨噬细胞单克隆抗体（MAbs），Am-3 K，PM-2K，X4，X14，Ber-MAC 3，GHI/61，EBM/11和KP 1与各种动物组织（包括大鼠，豚鼠，兔，猫，狗，山羊，猪，牛，马和猴）的种间反应性。所有MAb均识别猴巨噬细胞。除EBM/11和KP 1外，大多数单克隆抗体均能检测到猪巨噬细胞。八种抗体中，AM-3 K表现出最广泛的种间反应性。除大鼠外，它与所有检查动物种属的巨噬细胞反应。Western印迹分析显示，在豚鼠，兔和人类之间的AM-3 K识别的抗原的相似性。其他抗人单克隆抗体在动物中表现出对巨噬细胞的不同反应模式。所有这些单克隆抗体在动物组织中的免疫染色模式与在人类中发现的相似，尽管一些单克隆抗体，如AM-3 K，EBM/11和X4，在动物中比在人类中显示出更有限的反应性。这些结果表明，一些抗人单核/巨噬细胞单克隆抗体也可用于免疫组织化学检测动物组织中的单核/巨噬细胞。其中，AM-3 K被认为是用于鉴定动物各种组织中的巨噬细胞的最有用的单克隆抗体。
We examined interspecies reactivities of eight anti-human monocyte/macrophage monoclonal antibodies (MAbs), Am-3K, PM-2K, X4, X14, Ber-MAC3, GHI/61, EBM/11, and KP1, with various animal tissues including rats, guinea pigs, rabbits, cats, dogs, goats, pigs, bovines, horses, and monkeys. All MAbs recognized monkey macrophages. Pig macrophages were detected by most MAbs except for EBM/11 and KP1. Of the eight antibodies, AM-3K showed the widest interspecies reactivity. It reacted with macrophages of all animal species examined, except for rats. Western blot analysis revealed a similarity in the antigens recognized by AM-3K among guinea pigs, rabbits, and humans. Other anti-human MAbs demonstrated distinct reactive patterns against macrophages in animals. The immunostaining patterns of all of these MAbs in animal tissues were similar to those found in humans, although some MAbs, such as AM-3K, EBM/11, and X4, displayed more restricted reactivity in animals than in humans. These results indicate that some anti-human monocyte/macrophage MAbs are also available for immunohistochemical detection of monocyte/macrophages in animal tissues. Among them, AM-3K is considered to be the most useful MAb for identifying macrophages in various tissues of animals.