Analysis of chicken serum proteome and differential protein expression during development in single-comb White Leghorn hens
Analysis of chicken serum proteome and differential protein expression during development in single-comb White Leghorn hens
复制标题
DOI：
10.1002/pmic.200500410
复制
发表时间：
2006-04-01
期刊：
影响因子：
3.4
通讯作者：
Lee, WC
中科院分区：
文献类型：
--
作者：
Huang, SY;Lin, JH;Lee, WC
据信，血清中含有数千种不同的蛋白质，这些蛋白质要么是主动分泌的，要么是从各种血细胞或组织中泄漏出来的。探索血清中的蛋白质组成可能会加速发现牲畜物种特定经济性状的新型蛋白质生物标志物。本研究分析了血清蛋白组成，建立了 2-DE 参考图，并监测了单冠白来亨鸡孵化后 8、19 和 23 周的蛋白质动态。通过 MALDI-TOF MS 分析了总共 119 个 CBB 染色和 315 个银染色的血清蛋白点。其中，98 个 CBB 染色和 94 个银染色蛋白质点与现有的鸡肉蛋白质显着匹配。鉴定出的斑点代表蛋鸡血清中 30 种独特的蛋白质。为了比较发育过程中的蛋白质表达，使用 Melanie 3 软件通过相对点体积对 47 个蛋白质点的表达水平进行定量。随着母鸡日龄的增加，10个蛋白点增加，3个蛋白点减少。先前的研究表明，其中一些蛋白质在鸡蛋生产中发挥着关键作用。身份未知的差异表达蛋白将成为进一步探索其在蛋鸡产蛋中的作用的有价值的候选者。
Serum is believed to harbor thousands of distinct proteins that are either actively secreted or leak from various blood cells or tissues. Exploring protein composition in serum may accelerate the discovery of novel protein biomarkers for specific economic traits in livestock species. This study analyzed serum protein composition to establish a 2-DE reference map, and monitored protein dynamics of single-comb White Leghorn hens at 8, 19 and 23 weeks after hatching. A total of 119 CBB-stained and 315 silver-stained serum protein spots were analyzed by MALDI-TOF MS. Of these, 98 CBB-stained and 94 silver-stained protein spots were significantly matched to existing chicken proteins. The identified spots represented 30 distinctive proteins in the serum of laying hens. To compare protein expression during development, expression levels of 47 protein spots were quantified by relative spot volume with Melanie 3 software. Ten protein spots increased and 3 protein spots decreased as hen age increased. Previous research has suggested that some of these proteins play critical roles in egg production. The differentially expressed proteins with unknown identities will be valuable candidates for further explorations of their roles in egg production of laying hens.