Microscopic optical coherence tomography (mOCT) at 600 kHz for 4D volumetric imaging and dynamic contrast
Microscopic optical coherence tomography (mOCT) at 600 kHz for 4D volumetric imaging and dynamic contrast
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DOI：
10.1364/boe.425001
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发表时间：
2021-10-01
影响因子：
3.4
通讯作者：
Schulz-Hildebrandt, Hinnerk
中科院分区：
文献类型：
--
作者：
Muenter, Michael;Pieper, Mario;Schulz-Hildebrandt, Hinnerk
具有微观分辨率的动态过程的体积成像在生物医学研究和临床诊断中具有巨大的潜力。光学相干断层扫描 (OCT) 使用超连续谱光源和高数值孔径 (NA) 物镜，可实现微观分辨率，非常适合对生物组织的细胞和亚细胞结构进行成像。目前，显微OCT（mOCT）的成像速度受到光谱仪相机线扫描速率的限制，范围为30至250 kHz。这对于体内动态过程的体积成像来说不够快，并且限制了内窥镜的应用。使用新型 CMOS 相机，我们展示了快速 3 维 OCT 成像，每秒 600,000 次 A 扫描，轴向分辨率为 1.8 μm，横向分辨率为 1.1 μm。改进的速度用于离体小鼠气管中纤毛运动和颗粒运输的成像。此外，我们通过评估记录的体积而不是正面平面或 B 扫描来演示动态对比度 OCT。高速体积 mOCT 将能够校正整体组织运动，并且是在体内应用动态对比 mOCT 的先决条件。随着成像速度和柔性内窥镜集成度的进一步提高，体积 mOCT 可用于补充或部分替代活检。 (c) 2021 年美国光学协会根据 OSA 开放获取出版协议的条款
Volumetric imaging of dynamic processes with microscopic resolution holds a huge potential in biomedical research and clinical diagnosis. Using supercontinuum light sources and high numerical aperture (NA) objectives, optical coherence tomography (OCT) achieves microscopic resolution and is well suited for imaging cellular and subcellular structures of biological tissues. Currently, the imaging speed of microscopic OCT (mOCT) is limited by the line-scan rate of the spectrometer camera and ranges from 30 to 250 kHz. This is not fast enough for volumetric imaging of dynamic processes in vivo and limits endoscopic application. Using a novel CMOS camera, we demonstrate fast 3-dimensional OCT imaging with 600,000 A-scans/s at 1.8 mu m axial and 1.1 mu m lateral resolution. The improved speed is used for imaging of ciliary motion and particle transport in ex vivo mouse trachea. Furthermore, we demonstrate dynamic contrast OCT by evaluating the recorded volumes rather than en face planes or B-scans. High-speed volumetric mOCT will enable the correction of global tissue motion and is a prerequisite for applying dynamic contrast mOCT in vivo. With further increase in imaging speed and integration in flexible endoscopes, volumetric mOCT may be used to complement or partly replace biopsies. (c) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement