Distribution and in situ abundance of sulfate-reducing bacteria in diverse marine hydrocarbon seep sediments
Distribution and in situ abundance of sulfate-reducing bacteria in diverse marine hydrocarbon seep sediments
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DOI：
10.1111/j.1462-2920.2012.02832.x
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发表时间：
2012-10-01
影响因子：
5.1
通讯作者：
Knittel, Katrin
中科院分区：
文献类型：
--
作者：
Kleindienst, Sara;Ramette, Alban;Knittel, Katrin
海洋天然气和碳氢化合物渗漏是硫酸盐还原的热点，这种还原是由甲烷、其他短链烷烃或复杂的碳氢化合物混合物驱动的。本研究采用催化报告沉积荧光原位杂交技术（CARD-FISH）研究了8种油气渗漏中硫酸盐还原菌（SRB）的全球分布和丰度。大多数三角洲变形菌被划分为特定的SRB类群，气体渗漏为83 +/- 14%，烃渗漏为61 +/- 35%，表明所使用的探针集足以用于海洋SRB分类。统计分析表明，生境类型和沉积物深度对SRB丰度和分布有显著影响。Desulfosarcina/Desulfococcus （DSS）进化支的成员在所有位点均占主导地位。我们的数据表明，存在许多多样性和高度特化的低丰度DSS物种，而不是单一的丰富亚群。此外，未培养的深分支三角洲变形菌群SEEP-SRB2在所有位点均以高丰度普遍存在。SEEP-SRB2成员要么与贝壳型ANME-2/SEEP-SRB2联合体中的甲烷营养古菌有新的关联，要么与黑海微生物席中的ANME-1古菌有关联，要么以单细胞形式存在。另外两个未培养的组sepp - srb3和sepp - srb4优先在泥火山表面沉积物中检测到。
Marine gas and hydrocarbon seeps are hot spots of sulfate reduction which is fuelled by methane, other short-chain alkanes or a complex mixture of hydrocarbons. In this study, we investigated the global distribution and abundance of sulfate-reducing bacteria (SRB) in eight gas and hydrocarbon seeps by catalysed reporter deposition fluorescence in situ hybridization (CARD-FISH). The majority of Deltaproteobacteria were assigned to specific SRB groups, i.e. 83 +/- 14% at gas seeps and 61 +/- 35% at hydrocarbon seeps, indicating that the probe set used was sufficient for classification of marine SRB. Statistical analysis showed that SRB abundance and distribution were significantly influenced by habitat type and sediment depth. Members of the Desulfosarcina/Desulfococcus (DSS) clade strongly dominated all sites. Our data indicated the presence of many diverse and highly specialized DSS species of low abundance rather than a single abundant subgroup. In addition, SEEP-SRB2, an uncultured deep-branching deltaproteobacterial group, was ubiquitously found in high abundances at all sites. SEEP-SRB2 members occurred either in a novel association with methanotrophic archaea in shell-type ANME-2/SEEP-SRB2 consortia, in association with ANME-1 archaea in Black Sea microbial mats or as single cells. Two other uncultured groups, SEEP-SRB3 and SEEP-SRB4, were preferentially detected in surface sediments from mud volcanoes.