Prevalence and characterization of Shiga toxin-producing Escherichia coli isolated from cattle, food, and children during a one-year prospective study in France
Prevalence and characterization of Shiga toxin-producing Escherichia coli isolated from cattle, food, and children during a one-year prospective study in France
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DOI：
10.1128/jcm.38.3.1023-1031.2000
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发表时间：
2000-03-01
影响因子：
9.4
通讯作者：
Forestier, C
中科院分区：
文献类型：
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作者：
Pradel, N;Livrelli, V;Forestier, C
在对法国中部产志贺毒素大肠杆菌（STEC）流行率进行为期1年的调查期间，用PCR方法对2143份样本进行了志贺毒素编码基因的调查。在克莱蒙费朗屠宰场采集的471头健康牛粪便样本中，共有330头（70%）呈sh基因阳性，411头牛肉样本中有47头（11%），603头奶酪样本中有60头（10%），658头有或无腹泻住院儿童粪便样本中有19头（3%）呈sh基因阳性。从34%的牛粪便（162份/ 471份）、4%的牛肉样本（16份/ 411份）、1%的奶酪样本（5份/ 603份）和1.5%的粪便样本（10份/ 658份）中分离出产志贺毒素大肠杆菌菌株。在分离的220株产志在大肠杆菌菌株中，34株（15%）携带stx(1)基因，116株（53%）携带stx(2)基因，70株（32%）同时携带stx(1)和stx(2)基因。然而，32个（14.5%）对Vero细胞没有细胞毒性。220株中有12株（5%）存在eae基因，与stx(1)基因和儿童分离株显著相关。220株中有102株（46%）与ehxA同源。在186株可分型分离株中，有102株（55%）属于O157:H7血清型，其中只有1株（占186株牛产STEC分离株的0.5%）属于O157:H7血清型，分别为：OX3:H2、O113:H21、O113:H4、OX3:H21、O6:H10、OX178:H19、O171:H2、O46:H38、O172:H21、O22:H16。我们发现，从牛、牛肉和奶酪中分离出的大多数产志毒素大肠杆菌不太可能对人类具有致病性，并且本研究中从儿童中分离出的产志毒素大肠杆菌菌株可能不会导致腹泻病。最后，在同一地理区域与溶血性尿毒症综合征相关的菌株被证明属于牛库中发现的产志贺毒素大肠杆菌群体的特定亚群。
During a 1-year survey of Shiga toxin-producing Escherichia coli (STEC) prevalence in central France, 2,143 samples were investigated by PCR for Shiga toxin-encoding genes. A total of 330 (70%) of 471 fecal samples collected from healthy cattle at the Clermont-Ferrand slaughterhouse, 47 (11%) of 411 beef samples, 60 (10%) of 603 cheese samples, and 19 (3%) of 658 stool specimens from hospitalized children with and without diarrhea were positive for the sh gene(s). A STEC strain was isolated from 34% (162 of 471) of bovine feces, 4% (16 of 411) of beef samples, 1% (5 of 603) of cheese samples, and 1.5% (10 of 658) of stool specimens. Of the 220 STEC strains isolated, 34 (15%) harbored the stx(1) gene, 116 (53%) harbored the stx(2) gene, and 70 (32%) carried both the stx(1) and stx(2) genes. However, 32 (14.5%) were not cytotoxic for Vero cells. The eae gene, found in 12 (5%) of the 220 strains, was significantly associated with the stx(1) gene and with isolates from children. Sequences homologous to ehxA were found in 102 (46%) of the 220 strains. Thirteen serotypes, OX3:H2, O113:H21, O113:H4, OX3:H21, O6:H10, OX178:H19, O171:H2, O46:H38, O172:H21, O22:H16, O91:H10, O91:H21, and O22:H8, accounted for 102 (55%) of 186 typeable isolates, and only one strain (0.5% of the 186 STEC isolates from cattle), belonged to the O157:H7 serotype. We showed that the majority of the STEC isolates from cattle, beef, and cheese were not likely to be pathogenic for humans and that the STEC strains isolated from children in this study were probably not responsible for diarrheal disease. Finally, the strains associated with hemolytic-uremic syndrome in the same geographical area were shown to belong to particular subsets of the STEC population found in the bovine reservoir.