Immunohistochemical detection of uPA, tPA, and PAI-1 in a stasis-induced deep vein thrombosis model and its application to thrombus age estimation
Immunohistochemical detection of uPA, tPA, and PAI-1 in a stasis-induced deep vein thrombosis model and its application to thrombus age estimation
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DOI：
10.1007/s00414-012-0680-z
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发表时间：
2012-05-01
影响因子：
2.1
通讯作者：
Kondo, Toshikazu
中科院分区：
文献类型：
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作者：
Nosaka, Mizuho;Ishida, Yuko;Kondo, Toshikazu
我们使用小鼠下腔静脉 (IVC) 结扎形成的静脉血栓，通过免疫组织化学方法检测了尿激酶型纤溶酶原激活剂 (uPA)、组织型纤溶酶原激活剂 (tPA) 和 1 型纤溶酶原激活剂抑制剂 (PAI-1) 的表达。在IVC连接后5、7和3天分别可以首先检测到uPA、tPA和PAI-1阳性细胞。从形态上看，7 天后 PAI-1 阳性细胞的数量显着高于 uPA 和 tPA 阳性细胞的数量。在所有 10-21 天的血栓样本中，uPA/PAI-1 和 tPA/PAI-1 比率分别 > 0.1 和 > 0.2。相比之下，所有 1-7 天的血栓样本的 uPA/PAI-1 均小于 0.1，tPA/PAI-1 比率均小于 0.2。这些结果表明，uPA/PAI-1 > 0.1 和 tPA/PAI-1 > 0.2 表明年龄为 10 天或以上。此外，在 10 天龄的 5 个样本中，有 4 个样本的 uPA/PAI-1 比率 < 0.3，其余样本的 uPA/PAI-1 为 0.32。所有 14-21 天的血栓均显示出大于 0.3 的值。因此，uPA/PAI-1 比率明显超过 0.3，强烈表明年龄超过 14 天。本研究表明，uPA、tPA 和 PAI-1 的免疫组织化学检测适合估计静脉血栓的年龄。
We immunohistochemically examined the expression of urokinase-type plasminogen activator (uPA), tissue-type plasminogen activator (tPA), and plasminogen-activator inhibitor type-1 (PAI-1) using venous thrombi developed by ligation of the inferior vena cava (IVC) in mice. The uPA-, tPA- and PAI-1-positive cells could be firstly detected 5, 7, and 3 days, respectively, after IVC ligation. Morphometrically, the number of PAI-1-positive cells was significantly higher than those of uPA- and tPA-positive cells at later than 7 days. In all of the thrombus samples aged 10-21 days, the uPA/PAI-1 and tPA/PAI-1 ratios were > 0.1 and > 0.2, respectively. In contrast, all of the thrombus samples aged 1-7 days had uPA/PAI-1 of < 0.1 and tPA/PAI-1 ratios of < 0.2. These findings implied that uPA/PAI-1 of > 0.1 and tPA/PAI-1 of > 0.2 indicated an age of 10 days or more. Moreover, in four of five samples aged 10 days, uPA/PAI-1 ratios were < 0.3, and the remaining one had uPA/PAI-1 of 0.32. All thrombi aged 14-21 days showed values greater than 0.3. Thus, uPA/PAI-1 ratios, markedly exceeding 0.3, strongly indicated an age of more than 14 days. The present study demonstrated that the immunohistochemical detection of uPA, tPA, and PAI-1 was suitable to estimate the age of venous thrombi.