Phytosterol esters attenuate hepatic steatosis in rats with non-alcoholic fatty liver disease rats fed a high-fat diet.
Phytosterol esters attenuate hepatic steatosis in rats with non-alcoholic fatty liver disease rats fed a high-fat diet.
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植物甾醇酯可减轻高脂饮食的非酒精性脂肪肝大鼠的肝脏脂肪变性
DOI：
10.1038/srep41604
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发表时间：
2017-02-07
影响因子：
4.6
通讯作者：
Yan H
中科院分区：
文献类型：
--
作者：
Song L;Qu D;Zhang Q;Jiang J;Zhou H;Jiang R;Li Y;Zhang Y;Yan H
鉴于用于NAFLD治疗的药物的副作用，迫切需要鉴定新的和有效的天然化合物来预防NAFLD。在本研究中，植物甾醇酯（PSE）对NAFLD的影响进行了探讨。将成年SD大鼠随机分为5组：正常饮食组（NC）、高脂饮食组（HF）、PSE低、中、高剂量治疗加高脂饮食组（PSEL、PSEM和PSEH）。结果表明，PSEL组的LDL-C水平和三个PSE组的肝脏TG、TC和FFA水平均显著降低。值得注意的是，通过PSE干预，尿酸（UA）水平显著降低。PSEs干预组通过抑制TGF-β、IL-6、IL-10和CRP等细胞因子，减轻肝脏炎症应激。PSE处理还通过调节SOD和XOD的活性，降低MDA含量，改善氧化状态。PSE的这些作用可能与其在mRNA和蛋白水平上调节肝组织TGF-β1、TGF-β2、TNF-α、UCP-2、PPAR-α和PPAR-γ的表达有关。这项研究的结果表明，PSE可用作治疗剂，用于预防和发展NAFLD和高尿酸血症是由高脂肪饮食消费引起的。
Given the adverse effects of drugs used for NAFLD treatment, identifying novel and effective natural compound to prevent NAFLD is urgently needed. In the present study, the effects of phytosterol esters (PSEs) on NAFLD were explored. Adult SD rats were randomized into five groups: normal chow diet (NC), high-fat diet (HF), low-, medium- and high-dose PSE treatment plus high-fat diet groups (PSEL, PSEM, and PSEH). Our results showed that the levels of LDL-C in the PSEL group and hepatic TG, TC, and FFA in the three PSEs groups were significantly decreased. Notably, the uric acid (UA) level was significantly decreased by PSEs intervention. The hepatic inflammatory stress was ameliorated via the inhibition of the cytokines, including TGF-β, IL-6, IL-10 and CRP in the PSEs intervention groups. Further, the oxidative status was improved by PSE treatment through adjusting the enzyme activity (SOD and XOD) and decreasing the MDA level. These beneficial effects of PSE may have been partly due to its regulation on the expression of TGF-β1, TGF-β2, TNF-α, UCP-2, PPAR-α and PPAR-γ in hepatic tissue at both mRNA and protein level. The results of this study suggest that PSEs may be used as therapeutic agents for the prevention and progression of NAFLD and that hyperuricemia is induced by high-fat diet consumption.