The calsyntenins - A family of postsynaptic membrane proteins with distinct neuronal expression patterns
The calsyntenins - A family of postsynaptic membrane proteins with distinct neuronal expression patterns
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DOI：
10.1006/mcne.2002.1181
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发表时间：
2002-11-01
影响因子：
3.5
通讯作者：
Sonderegger, P
中科院分区：
文献类型：
--
作者：
Hintsch, G;Zurlinden, A;Sonderegger, P
我们已经确定了两种新的突触后膜蛋白，它们在细胞外部分与calsyntenin-1高度相似，但在细胞质部分差异很大。最近在我们的实验室中已经鉴定出钙合蛋白-1是一种突触后膜蛋白，其具有高度酸性的细胞质片段，具有推定的Ca 2+结合能力（沃格特et al.，2001，Mol. Cell. Neurosci. 17：151-166）。基于它们与calsyntenin-1的结构相似性，我们分别将新蛋白质称为calsyntenin-2和-3。免疫电镜观察发现，Calsyntenin蛋白家族定位于兴奋性中枢神经系统（CNS）突触的突触后膜。原位杂交分析表明，calsyntenin-1是丰富的，在大多数神经元的中枢神经系统的表达水平相对较小的变化。Calsyntenin-2和Calsyntenin-3的表达变化更大，在GABA能神经元中表达水平最高。基于它们不同的表达模式和它们的细胞质片段的差异，我们建议这三种calsyntenins在兴奋性突触传递中具有细胞类型特异性的功能作用。
We have identified two novel postsynaptic membrane proteins that are highly similar to calsyntenin-1 in their extracellular parts but vary considerably in their cytoplasmic segment. Calsyntenin-1 has recently been identified in our lab as a postsynaptic membrane protein with a highly acidic cytoplasmic segment with putative Ca2+-binding capacity (Vogt et al., 2001, Mol. Cell. Neurosci. 17:151-166). Based on their structural similarity to calsyntenin-1, we have called the novel proteins calsyntenin-2 and -3, respectively. By immunoelectron microscopy, the calsyntenin protein family was localized in the postsynaptic membrane of excitatory central nervous system (CNS) synapses. In situ hybridization analysis revealed that calsyntenin-1 was abundant in most neurons of the CNS with relatively little variation in its expression level. Calsyntenin-2 and -3 expressions varied much more with highest levels in GABAergic neurons. Based on their distinct expression patterns and the differences in their cytoplasmic segments, we suggest a cell-type-specific functional role for the three calsyntenins in excitatory synaptic transmission.