Identification of a peroxisomal targeting signal at the carboxy terminus of firefly luciferase.
Identification of a peroxisomal targeting signal at the carboxy terminus of firefly luciferase.
复制标题
DOI：
10.1083/jcb.105.6.2923
复制
发表时间：
1987-12
期刊：
影响因子：
--
通讯作者：
Subramani S
中科院分区：
文献类型：
--
作者：
Gould SG;Keller GA;Subramani S
蛋白质跨内质网、线粒体和叶绿体的跨膜转运已被证明是由转运的蛋白质中存在的靶向信号所介导的。为了测试蛋白质到过氧酶体的运输是否也是由多肽靶向信号介导的，我们研究了萤火虫荧光素酶基因，它编码一种在昆虫和哺乳动物细胞中运输到过氧酶体的蛋白质。我们已经确定了荧光素酶的两个区域，这两个区域是将这种蛋白质运输到过氧化体中所必需的。我们证明其中的一个，区域II，代表了一个过氧化体靶向信号，因为它既是引导胞质蛋白进入过氧化体的必要条件，也是充分条件。该信号长度不超过12个氨基酸，位于荧光素酶的极端羧基末端。因此，靶向信号的位置不同于负责跨内质网、叶绿体或线粒体膜运输的信号的主要氨基末端位置。
Translocation of proteins across membranes of the endoplasmic reticulum, mitochondrion, and chloroplast has been shown to be mediated by targeting signals present in the transported proteins. To test whether the transport of proteins into peroxisomes is also mediated by a peptide targeting signal, we have studied the firefly luciferase gene that encodes a protein transported to peroxisomes in both insect and mammalian cells. We have identified two regions of luciferase which are necessary for transport of this protein into peroxisomes. We demonstrate that one of these, region II, represents a peroxisomal targeting signal because it is both necessary and sufficient for directing cytosolic proteins to peroxisomes. The signal is no more than twelve amino acids long and is located at the extreme carboxy-terminus of luciferase. The location of the targeting signal for translocation across the peroxisomal membrane therefore differs from the predominantly amino-terminal location of signals responsible for transport across the membranes of the endoplasmic reticulum, chloroplast, or mitochondrion.