Diversity in butane monooxygenases among butane-grown bacteria.
Diversity in butane monooxygenases among butane-grown bacteria.
复制标题
丁烷生长的细菌中丁烷单加氧酶的多样性。
DOI：
10.1128/aem.65.10.4586-4593.1999
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发表时间：
1999
影响因子：
4.4
通讯作者：
Arp,DJ
中科院分区：
文献类型：
--
作者：
Hamamura,N;Storfa,RT;Semprini,L;Arp,DJ
从生理水平比较了丁烷生长假单胞菌、母牛分枝杆菌JOB5和环境菌株CF8的丁烷单加氧酶。结果表明，这些细菌中存在丁烷单加氧酶。(I)丁烷的降解需要氧气。(Ii)丁烷降解过程中产生正丁醇。(3)乙炔抑制丁烷氧化和1-丁醇生成。对已知的单加氧酶失活剂乙烯和抑制剂烯丙基硫脲(ATU)的反应，区分了这三种细菌对丁烷的降解。乙烯不可逆钝化丁烷氧化。但不是由M。疫苗或CF8。相反，只有CF8的丁烷氧化被ATU强烈地抑制。在所有三株丁烷生长的细菌中，都在[14C]乙炔存在下标记了特定的多肽。三种细菌的[14C]乙炔标记图谱不同。乳酸盐生长的CF8和P的暴露。Butanovora和葡萄糖生长菌M.Vaaeto丁烷可诱导丁烷氧化活性和特异的乙炔结合多肽。三种细菌均能氧化氨气，丁酸假单胞菌将氨氧化为羟胺，而CF8和M。疫苗产生的亚硝酸盐。这三种细菌都将乙烯氧化成环氧乙烷。任何细菌都没有检测到甲烷氧化。结果表明，在丁烷生长的P中存在三种不同的丁烷单加氧酶。Butanovora、M.vaccae和CF8。
Butane monooxygenases of butane-grownPseudomonas butanovora,Mycobacterium vaccaeJOB5, and an environmental isolate, CF8, were compared at the physiological level. The presence of butane monooxygenases in these bacteria was indicated by the following results. (i) O2was required for butane degradation. (ii) 1-Butanol was produced during butane degradation. (iii) Acetylene inhibited both butane oxidation and 1-butanol production. The responses to the known monooxygenase inactivator, ethylene, and inhibitor, allyl thiourea (ATU), discriminated butane degradation among the three bacteria. Ethylene irreversibly inactivated butane oxidation byP. butanovorabut not byM. vaccaeor CF8. In contrast, butane oxidation by only CF8 was strongly inhibited by ATU. In all three strains of butane-grown bacteria, specific polypeptides were labeled in the presence of [14C]acetylene. The [14C]acetylene labeling patterns were different among the three bacteria. Exposure of lactate-grown CF8 andP. butanovoraand glucose-grownM. vaccaeto butane induced butane oxidation activity as well as the specific acetylene-binding polypeptides. Ammonia was oxidized by all three bacteria.P. butanovoraoxidized ammonia to hydroxylamine, while CF8 andM. vaccaeproduced nitrite. All three bacteria oxidized ethylene to ethylene oxide. Methane oxidation was not detected by any of the bacteria. The results indicate the presence of three distinct butane monooxygenases in butane-grownP. butanovora,M. vaccae, and CF8.