Distinct regulators for Plk1 activation in starfish meiotic and early embryonic cycles
Distinct regulators for Plk1 activation in starfish meiotic and early embryonic cycles
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DOI：
10.1093/emboj/cdg535
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发表时间：
2003-10-15
期刊：
影响因子：
11.4
通讯作者：
Kishimoto, T
中科院分区：
文献类型：
--
作者：
Okano-Uchida, T;Okumura, E;Kishimoto, T
Polo样激酶，PLK，在调节有丝分裂中有多种作用。特别是，Plk1被认为是一种触发蛋白，在细胞周期蛋白B-CDc2被激活之前将其磷酸化并激活，从而启动其激活。然而，Plk1激活的上游调控仍不清楚。在这里，我们通过海星的减数分裂和早期胚胎周期研究了Plk1和CDc2之间的相互作用。在减数分裂I期、减数分裂II期和胚胎M期，细胞周期蛋白B-cdc2、MAPK、细胞周期蛋白B-cdc2和/或细胞周期蛋白A-cdc2、细胞周期蛋白A-cdc2分别是Plk1激活的上游调控因子，表明Plk1不是减数分裂重新启动的触发蛋白。当细胞周期蛋白B-CDC2的激活需要Plk1时，Plk1的作用主要通过抑制Myt1而不是通过激活CDC25来实现。我们认为Plk1可以被Cyclin A-或Cyclin B-CDc2激活，其主要靶点是Myt1。
The Polo-like kinase, Plk, has multiple roles in regulating mitosis. In particular, Plk1 has been postulated to function as a trigger kinase that phosphorylates and activates Cdc25C prior to the activation of cyclin B-Cdc2 and thereby initiates its activation. However, the upstream regulation of Plk1 activation remains unclear. Here we have studied the interplay between Plk1 and Cdc2 through meiotic and early embryonic cycles in starfish. Distinct kinases, cyclin B-Cdc2, MAPK along with cyclin B- and/or cyclin A-Cdc2 and cyclin A-Cdc2, were unique upstream regulators for Plk1 activation at meiosis I, meiosis II and embryonic M-phase, respectively, indicating that Plk1 is not the trigger kinase at meiotic reinitiation. When Plk1 was required for cyclin B-Cdc2 activation, the action of Plk1 was mediated primarily through suppression of Myt1 rather than through activation of Cdc25. We propose that Plk1 can be activated by either cyclin A- or cyclin B-Cdc2, and its primary target is Myt1.